4O38 Chain A
Cyclin-G-associated kinase (GAK)
Active — 77.5%DFG-inαC-in
Resolution
2.1 Å
R-value
0.166
Predicted activity confidence77.5%
Structure info
Alternate conformationB
Missing atoms0
Missing residues1
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
QSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKESRGPLSCDTVLKIFYQTCRAVQHHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPLYSNFPIGEKQDIWALGCILYLLCFRQHPFYSIPPHDTQYTVFHSLIRALQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQ
UniProt reference sequence
LRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQE
Aligned reference sequence
LRVR----------------RVLAEGG----------------FAFVYEAQD--VG------------------------------------SGREYALKRLL--SNEEEK----------------------------NRAIIQEVCFMKKL--S-----------------------GHPNIVQFCSAASIGK--EESDTGQA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EFLLLTELCK---------------GQLVEFLKK--MESRG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLSCDTVLKIFYQTCRAVQHMHRQ--KP-----------------------------------------PIIHRDL-----KVENLLLSN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGTIKLCDFGSATTISHYPDY--SWSAQRRALVEEE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ITRNTTPMYRTPEIIDL--YSN---------------------------FPIG-EKQDIWALGCILYLLCFR-----------------QHPFED------------------------------------------------------GAKLRIVNG--KYSIPPHDTQY----------------------------------------------------------------------------------------------------TVFHSLIRAMLQVNPEERLS--------------IAEVVHQLQE
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LRVR----------------RVLAEGG----------------FAFVYEAQD--VG------------------------------------SGREYALKRLL--SNEEEK----------------------------NRAIIQEVCF-KKL--S-----------------------GHPNIVQFCSAASIGK--EESDTGQA----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EFLLLTELCK---------------GQLVEFLKK---ESRG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLSCDTVLKIFYQTCRAVQH-HRQ--KP-----------------------------------------PIIHRDL-----KVENLLLSN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGTIKLCDFGSATTISHYP------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------L--YSN---------------------------FPIG-EKQDIWALGCILYLLCFR-----------------QHPF--------------------------------------------------------------------YSIPPHDTQY----------------------------------------------------------------------------------------------------TVFHSLIRA-LQVNPEERLS--------------IAEVVHQLQE
Activation segment
DFGSATTISHYP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Binding pocket
RVLAEGGFAFVYEYALKRLAIIQEVCF_KKLSPNIVQFCSALLLTELCK_GQLVEFLKKHRQKPPIIHRDLKVENLLLLCDFGSA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4O38, Chain A