4LUD Chain B
Tyrosine-protein kinase HCK (HCK)
Inactive — 0.5%DFG-inαC-outType1 · SK8
Resolution
2.85 Å
R-value
0.215
Predicted activity confidence0.5%
Structure info
Alternate conformation—
Missing atoms4
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
RIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETSYSLSVRDYDPRQGDTVKHYKITLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQEE
UniProt reference sequence
LKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGMSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDD
Aligned reference sequence
LKLE----------------KKLGAGQ----------------FGEVWMATY--N-------------------------------------KHTKVAVKTMK--PGSMS-----------------------------VEAFLAEANVMKTL--------------------------QHDKLVKLHAVVTKE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEFMAK--------------GSLLDFLKS--DEGS-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KQPLPKLIDFSAQIAEGMAFIEQR---------------------------------------------NYIHRDL-----RAANILVSA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLVCKIADFGLARVIEDNEYT--AR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGAKFPIKWTAPEAINF--------------------------------GSFT-IKSDVWSFGILLMEIVTY--G--------------RIPYPG--------------------------------------------------MSNPEVIRALERG--YRMPRPENCP-----------------------------------------------------------------------------------------------------EELYNIMMRCWKNRPEERPT--------------FEYIQSVLDD
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LKLE----------------KKLGAGQ----------------FGEVWMATY--N-------------------------------------KHTKVAVKTMK--PG--S-----------------------------VEAFLAEANVMKTL--------------------------QHDKLVKLHAVVTKE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEFMAK--------------GSLLDFLKS--DEGS-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KQPLPKLIDFSAQIAEGMAFIEQR---------------------------------------------NYIHRDL-----RAANILVSA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLVCKIADFGLARVI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIKWTAPEAINF--------------------------------GSFT-IKSDVWSFGILLMEIVTY--G--------------RIPYPG--------------------------------------------------MSNPEVIRALERG--YRMPRPENCP-----------------------------------------------------------------------------------------------------EELYNIMMRCWKNRPEERPT--------------FEYIQSVLDD
Activation segment
DFGLARVI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIKWTAPE
Binding pocket
KKLGAGQFGEVWMVAVKTMAFLAEANVMKTLQDKLVKLHAVYIITEFMAKGSLLDFLKSFIEQRNYIHRDLRAANILVIADFGLA
Ligand info
Orthosteric ligand
SK8
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4LUD, Chain B