4KIO Chain D
Tyrosine-protein kinase ITK/TSK (ITK)
Inactive — 0.3%DFG-inαC-inType1 · G6K
Resolution
2.18 Å
R-value
0.167
Predicted activity confidence0.3%
Structure info
Alternate conformationA
Missing atoms10
Missing residues1
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
GSVIDPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTISEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAEIAES
UniProt reference sequence
LTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIREGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRTQRGLFAAETLLGMCLDVCEGMAYLEEACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASTHVYQIMNHCWKERPEDRPAFSRLLRQLAE
Aligned reference sequence
LTFV----------------QEIGSGQ----------------FGLVHLGYW--L-------------------------------------NKDKVAIKTIR--EGAMS-----------------------------EEDFIEEAEVMMKL--------------------------SHPKLVQLYGVCLEQA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PICLVFEFMEH--------------GCLSDYLRT--QRG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LFAAETLLGMCLDVCEGMAYLEEA---------------------------------------------CVIHRDL-----AARNCLVGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NQVIKVSDFGMTRFVLDDQYT--SS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGTKFPVKWASPEVFSF--------------------------------SRYS-SKSDVWSFGVLMWEVFSE--G--------------KIPYEN--------------------------------------------------RSNSEVVEDISTG--FRLYKPRLAS-----------------------------------------------------------------------------------------------------THVYQIMNHCWKERPEDRPA--------------FSRLLRQLAE
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LTFV----------------QEIGSGQ----------------FGLVHLGYW--L-------------------------------------NKDKVAIKTI-------S-----------------------------EEDFIEEAEVMMKL--------------------------SHPKLVQLYGVCLEQA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PICLVFEFMEH--------------GCLSDYLRT--QRG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LFAAETLLGMCLDVCEGMAYLEEA---------------------------------------------CVIHRDL-----AARNCLVGE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NQVIKVSDFGM---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PVKWASPEVFSF--------------------------------SRYS-SKSDVWSFGVLMWEVFSE--G--------------KIPYEN--------------------------------------------------RSNSEVVEDISTG--FRLYKPRLAS-----------------------------------------------------------------------------------------------------THVYQIMNHCWKERPEDRPA--------------FSRLLRQLAE
Activation segment
DFGM---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PVKWASPE
Binding pocket
QEIGSGQFGLVHLVAIKTIDFIEEAEVMMKLSPKLVQLYGVCLVFEFMEHGCLSDYLRTYLEEACVIHRDLAARNCLVVSDFGM_
Ligand info
Orthosteric ligand
G6K
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4KIO, Chain D