4IDT Chain A
Mitogen-activated protein kinase kinase kinase 14 (MAP3K14)
Active — 87.4%DFG-inαC-inType1 · T28
Resolution
2.4 Å
R-value
0.193
Predicted activity confidence87.4%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
VEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHP
UniProt reference sequence
WATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNR
Aligned reference sequence
WATH--Q-------------LRLGRGS----------------FGEVHRMED--KQ------------------------------------TGFQCAVKKVR--L---------------------------------EVFRAEELMACAGL--------------------------TSPRIVPLYGAVREGP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WVNIFMELLEG--------------GSLGQLVKE--QG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLPEDRALYYLGQALEGLEYLHSR---------------------------------------------RILHGDV-----KADNVLLSS--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSHAALCDFGHAVCLQPDGLG--KSLLTG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYIPGTETHMAPEVVLG--------------------------------RSCD-AKVDVWSSCCMMLHMLNG-----------------CHPWTQ--------------------------------------------------FFRGPLCLKIASE--PPPVREIPPSCA---------------------------------------------------------------------------------------------------PLTAQAIQEGLRKEPIHRVS--------------AAELGGKVNR
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
WATH--Q-------------LRLGRGS----------------FGEVHRMED--KQ------------------------------------TGFQCAVKKVR--L---------------------------------EVFRAEELMACAGL--------------------------TSPRIVPLYGAVREGP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WVNIFMELLEG--------------GSLGQLVKE--QG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLPEDRALYYLGQALEGLEYLHSR---------------------------------------------RILHGDV-----KADNVLLSS--D-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GSHAALCDFGHAVCLQ-----------TG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYIPGTETHMAPEVVLG--------------------------------RSCD-AKVDVWSSCCMMLHMLNG-----------------CHPWTQ--------------------------------------------------FFRGPLCLKIASE--PPPVREIPPSCA---------------------------------------------------------------------------------------------------PLTAQAIQEGLRKEPIHRVS--------------AAELGGKVNR
Activation segment
DFGHAVCLQ-----------TG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYIPGTETHMAPE
Binding pocket
LRLGRGSFGEVHRCAVKKVFRAEELMACAGLTPRIVPLYGANIFMELLEGGSLGQLVKEYLHSRRILHGDVKADNVLLLCDFGHA
Ligand info
Orthosteric ligand
T28
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4IDT, Chain A