4IAD Chain A
cAMP-dependent protein kinase catalytic subunit alpha (PRKACA)
Active — 100.0%DFG-inαC-inATPlike · ADP
Resolution
1.9 Å
R-value
0.189
Predicted activity confidence100.0%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVINEKCGKEFTEF
UniProt reference sequence
FERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWF
Aligned reference sequence
FERI----------------KTLGTGS----------------FGRVMLVKH--KE------------------------------------TGNHYAMKILD--KQKVVKLKQ-------------------------IEHTLNEKRILQAV--------------------------NFPFLVKLEFSFKDNS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYMVMEYVPG--------------GEMFSHLRR--IG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSEPHARFYAAQIVLTFEYLHSL---------------------------------------------DLIYRDL-----KPENLLIDQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGYIQVTDFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WTLCGTPEYLAPEIILS--------------------------------KGYN-KAVDWWALGVLIYEMAAG-----------------YPPFFA--------------------------------------------------DQPIQIYEKIVSG--KVRFPSHFS------------------------------------------------------------------------------------------------------SDLKDLLRNLLQVDLTKRFG--NLKNG-------VNDIKNHKWF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FDRI----------------KTLGTGS----------------FGRVMLVKH--KE------------------------------------SGNHYAMKILD--KQKVVKLKQ-------------------------IEHTLNEKRILQAV--------------------------NFPFLVKLEFSFKDNS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYMVMEYVAG--------------GEMFSHLRR--IG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RF-EPHARFYAAQIVLTFEYLHSL---------------------------------------------DLIYRDL-----KPENLLIDQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGYIQVTDFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------W-LCGTPEYLAPEIILS--------------------------------KGYN-KAVDWWALGVLIYEMAAG-----------------YPPFFA--------------------------------------------------DQPIQIYEKIVSG--KVRFPSHFS------------------------------------------------------------------------------------------------------SDLKDLLRNLLQVDLTKRFG--NLKNG-------VNDIKNHKWF
Activation segment
DFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------W-LCGTPEYLAPE
Binding pocket
KTLGTGSFGRVMLYAMKILHTLNEKRILQAVNPFLVKLEFSYMVMEYVAGGEMFSHLRRYLHSLDLIYRDLKPENLLIVTDFGFA
Ligand info
Orthosteric ligand
ADP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4IAD, Chain A