4H3P Chain A
Mitogen-activated protein kinase 1 (MAPK1)
Active — 95.5%DFG-inαC-inATPlike · ANP
Resolution
2.3 Å
R-value
0.18
Predicted activity confidence95.5%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
PEMVRGQVFDVGPRYTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLAFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYLAQYYDPSDEPIAEAPFKFDMELDDLPKEKLKELIFEETARFQPGYR
UniProt reference sequence
YTNLSYIGEGAYGMVCSAYDNVNKVRVAIKKISPFEHQTYCQRTLREIKILLRFRHENIIGINDIIRAPTIEQMKDVYIVQDLMETDLYKLLKTQHLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLLNTTCDLKICDFGLARVADPDHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNADSKALDLLDKMLTFNPHKRIEVEQALAHPYL
Aligned reference sequence
YTNL----------------SYIGEGA----------------YGMVCSAYD--NV------------------------------------NKVRVAIKKIS--PFEHQTY---------------------------CQRTLREIKILLRF--------------------------RHENIIGINDIIRAPT--IEQMK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVQDLME---------------TDLYKLLKT--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLSNDHICYFLYQILRGLKYIHSA---------------------------------------------NVLHRDL-----KPSNLLLNT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCDLKICDFGLARVADPDHDH--TGFL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEYVATRWYRAPEIMLN--S-----------------------------KGYT-KSIDIWSVGCILAEMLSN-----------------RPIFPG--------------------------------------------------KHYLDQLNHILGI--LGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNAD-----------------------------------------------------------------------SKALDLLDKMLTFNPHKRIE--------------VEQALAHPYL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YTNL----------------SYIGEGA----------------YGMVCSAYD--NV------------------------------------NKVRVAIKKIS--PFEHQTY---------------------------CQRTLREIKILLAF--------------------------RHENIIGINDIIRAPT--IEQMK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVQDLME---------------TDLYKLLKT--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HLSNDHICYFLYQILRGLKYIHSA---------------------------------------------NVLHRDL-----KPSNLLLNT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCDLKICDFGLARVADPDH---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EYVATRWYRAPEIMLN--S-----------------------------KGYT-KSIDIWSVGCILAEMLSN-----------------RPIFPG--------------------------------------------------KHYLDQLNHILGI--LGSPSQEDLNCIINLKARNYLLSLPHKNKVPWNRLFPNAD-----------------------------------------------------------------------SKALDLLDKMLTFNPHKRIE--------------VEQALAHPYL
Activation segment
DFGLARVADPDH---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EYVATRWYRAPE
Binding pocket
SYIGEGAYGMVCSVAIKKIRTLREIKILLAFRENIIGINDIYIVQDLME_TDLYKLLKTYIHSANVLHRDLKPSNLLLICDFGLA
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4H3P, Chain A