4GL9 Chain A
Tyrosine-protein kinase JAK2 (JAK2)
Active — 98.6%DFG-inαC-inType1 · IZA
Resolution
3.9 Å
R-value
0.254
Predicted activity confidence98.6%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
QFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSNLRLIMEYLPYGSLRDYLQKHKERIDHKKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPVEFMRMIGNDKQGQMIVFHLIELLKSNGRLPRPEGCPDEIYVIMTECWNNNVSQRPSFRDLSLRVDQIRDSI
UniProt reference sequence
LIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNS
Aligned reference sequence
LIFN----------------ESLGQGT----------------FTKIFKGVR--REVGDYGQL-----------------------------HETEVLLKVLD--KAHRNY----------------------------SESFFEAASMMSKL--------------------------SHKHLVLNYGVCVCGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ENILVQEFVKF--------------GSLDTYLKK--NKN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CINILWKLEVAKQLAWAMHFLEEN---------------------------------------------TLIHGNV-----CAKNILLIR--EEDRKTGN----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PPFIKLSDPGISITVLPK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DILQERIPWVPPECIEN--P-----------------------------KNLN-LATDKWSFGTTLWEICSG--G--------------DKPLSA--------------------------------------------------LDSQRKLQFYEDR--HQLPAPKW-------------------------------------------------------------------------------------------------------AELANLINNCMDYEPDFRPS--------------FRAIIRDLNS
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LKFL----------------QQLGKG--------------------NFGSVE--MCRYDPLQD-----------------------------NTGEVVAVKKL--QHSTEE----------------------------HLRDFEREIEILKL--------------------------QHDNIVKYKG--VCYS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLRLIMEYLPY--------------GSLRDYLQK--HKE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RIDHKKLLQYTSQICKGMEYLGTK---------------------------------------------RYIHRDL-----ATRNILVEN--ENRVKIGD----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FGLTK---------VLPQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKEKVKEPGESPIFWYA--P-----------------------------ESLS-VASDVWSFGVVLYELFTY--I--------------EKSKSG--------------------------------------------------NDKQIELLKSNGR--LPRPEGCP-------------------------------------------------------------------------------------------------------DEIYVIMTECWNNNVSQRPS--------------FRDQIRD-SI
Activation segment
-------VLPQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DKEKVKEPGESPI
Binding pocket
QQLGKGNFGSVEMVAVKKLDFEREIEILKSLQDNIVKYKGVRLIMEYLPYGSLRDYLQKYLGTKRYIHRDLATRNILVIGDFGLT
Ligand info
Orthosteric ligand
IZA
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4GL9, Chain A