4FR4 Chain B
Serine/threonine-protein kinase 32A (STK32A)
Active — 99.8%DFG-inαC-inType1 · STU
Resolution
2.29 Å
R-value
0.206
Predicted activity confidence99.8%
Structure info
Alternate conformationA
Missing atoms3
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
ENEDVNFDHFEILRAIGKGSFGKVCIVQKNDTKKMYAMKYMNKQKCVERNEVRNVFKELQIMQGLEHPFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFKEETVKLFICELVMALDYLQNQRIIHRDMKPDNILLDEHGHVHITDFNIAAMLPRETQITTMAGTKPYMAPEMFSSRKGAGYSFAVDWWSLGVTAYELLRGRRPYHIRSSTSSKEIVHTFETTVVTYPSAWSQEMVSLLKKLLEPNPDQRFSQLSDVQNFPYMNDINWDAVFQKRLIPGFIPNKGRLNCDPTFELEEMILESKKKEKDMRKCDSSQTCLLQEHLDSVQKEFIIFNREKVNRDFNK
UniProt reference sequence
FEILRAIGKGSFGKVCIVQKNDTKKMYAMKYMNKQKCVERNEVRNVFKELQIMQGLEHPFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFKEETVKLFICELVMALDYLQNQRIIHRDMKPDNILLDEHGHVHITDFNIAAMLPRETQITTMAGTKPYMAPEMFSSRKGAGYSFAVDWWSLGVTAYELLRGRRPYHIRSSTSSKEIVHTFETTVVTYPSAWSQEMVSLLKKLLEPNPDQRFSQLSDVQNFPYM
Aligned reference sequence
FEIL----------------RAIGKGS----------------FGKVCIVQK--ND------------------------------------TKKMYAMKYMN--KQKCVERNE-------------------------VRNVFKELQIMQGL--------------------------EHPFLVNLWYSFQDEE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMFMVVDLLLG--------------GDLRYHLQQ--NV-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HFKEETVKLFICELVMALDYLQNQ---------------------------------------------RIIHRDM-----KPDNILLDE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HGHVHITDFNIAAMLPRETQI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTMAGTKPYMAPEMFSS--RKG---------------------------AGYS-FAVDWWSLGVTAYELLRG-----------------RRPYHI--RSS---------------------------------------------TSSKEIVHTFETT--VVTYPSAWS------------------------------------------------------------------------------------------------------QEMVSLLKKLLEPNPDQRFS--Q-----------LSDVQNFPYM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FEIL----------------RAIGKGS----------------FGKVCIVQK--ND------------------------------------TKKMYAMKYMN--KQKCVERNE-------------------------VRNVFKELQIMQGL--------------------------EHPFLVNLWYSFQDEE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMFMVVDLLLG--------------GDLRYHLQQ--NV-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HFKEETVKLFICELVMALDYLQNQ---------------------------------------------RIIHRDM-----KPDNILLDE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HGHVHITDFNIAAMLPRETQI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTMAGTKPYMAPEMFSS--RKG---------------------------AGYS-FAVDWWSLGVTAYELLRG-----------------RRPYHI--RSS---------------------------------------------TSSKEIVHTFETT--VVTYPSAWS------------------------------------------------------------------------------------------------------QEMVSLLKKLLEPNPDQRFS--Q-----------LSDVQNFPYM
Activation segment
DFNIAAMLPRETQI------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTMAGTKPYMAPE
Binding pocket
RAIGKGSFGKVCIYAMKYMNVFKELQIMQGLEPFLVNLWYSFMVVDLLLGGDLRYHLQQYLQNQRIIHRDMKPDNILLITDFNIA
Ligand info
Orthosteric ligand
STU
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4FR4, Chain B