4F9A Chain C
Cell division cycle 7-related protein kinase (CDC7)
Active — 63.0%DFG-outαC-inATPlike · ADP
Resolution
2.17 Å
R-value
0.208
Predicted activity confidence63.0%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
LAGVKKDIEKLYEAVPQLSNVFKIEDKIGEGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQSEAQQRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGWNEVPDEAYDLLDKLLDLNPASRITAEEALLHPFFKDM
UniProt reference sequence
FKIEDKIGEGTFSSVYLATAQLQVGPEEKIALKHLIPTSHPIRIAAELQCLTVAGGQDNVMGVKYCFRKNDHVVIAMPYLEHESFLDILNSLSFQEVREYMLNLFKALKRIHQFGIVHRDVKPSNFLYNRRLKKYALVDFGLAQGTHDTKIELLKFVQSEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGLSVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLTCDCYATDKVCSICLSRRQQVAPRAGTPGFRAPEVLTKCPNQTTAIDMWSAGVIFLSLLSGRYPFYKASDDLTALAQIMTIRGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHASHQPAISEKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVPDEAYDLLDKLLDLNPASRITAEEALLHPFF
Aligned reference sequence
FKIE----------------DKIGEGT----------------FSSVYLATA--QLQVG---------------------------------PEEKIALKHLI--PTSH------------------------------PIRIAAELQCLTVA--G-----------------------GQDNVMGVKYCFRKND--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HVVIAMPYLEH--------------ESFLDILN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLSFQEVREYMLNLFKALKRIHQF---------------------------------------------GIVHRDV-----KPSNFLYNR--R-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LKKYALVDFGLAQGTHDTKIE--LLKFVQSEAQQERCSQNKSHIITGNKIPLSGPVPKELDQQSTTKASVKRPYTNAQIQIKQGKDGKEGSVGLSVQRSVFGERNFNIHSSISHESPAVKLMKQSKTVDVLSRKLATKKKAISTKVMNSAVMRKTASSCPASLTCDCYATDKVCSICLSRRQQV-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------APRAGTPGFRAPEVLTK--C-----------------------------PNQT-TAIDMWSAGVIFLSLLSG--R--------------YPFYKA--------------------------------------------------SDDLTALAQIMTI--RGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLRGMDSSTPKLTSDIQGHASHQPAISEKTDHKASCLVQTPPGQYSGNSFKKGDSNSCEHCFDEYNTNLEGWNEVP--DEAYDLLDKLLDLNPASRIT--------------AEEALLHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FKIE----------------DKIGEGT----------------FSSVYLATA--QLQVG---------------------------------PEEKIALKHLI--PTSH------------------------------PIRIAAELQCLTVA--G-----------------------GQDNVMGVKYCFRKND--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HVVIAMPYLEH--------------ESFLDILN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLSFQEVREYMLNLFKALKRIHQF---------------------------------------------GIVHRDV-----KPSNFLYNR--R-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LKKYALVDFGLAQGTHDTKIE--LLKFVQSEAQQ-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RAGTPGFRAPEVLTK--C-----------------------------PNQT-TAIDMWSAGVIFLSLLSG--R--------------YPFYKA--------------------------------------------------SDDLTALAQIMTI--RGSRETIQAAKTFGKSILCSKEVPAQDLRKLCERLR-------------------------------------------------------------------GWNEVP--DEAYDLLDKLLDLNPASRIT--------------AEEALLHPFF
Activation segment
DFGLAQGTHDTKIE--LLKFVQSEAQQ-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RAGTPGFRAPE
Binding pocket
DKIGEGTFSSVYLIALKHLRIAAELQCLTVAGDNVMGVKYCVIAMPYLE_HESFLD__IRIHQFGIVHRDVKPSNFLYLVDFGLA
Ligand info
Orthosteric ligand
ADP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4F9A, Chain C