4CXA Chain A
Cyclin-dependent kinase 12 (CDK12)
Active — 77.8%DFG-inαC-inATPlike · ANP
Resolution
3.15 Å
R-value
0.224
Predicted activity confidence77.8%
Structure info
Alternate conformation—
Missing atoms19
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
DWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVNMKEIVTDKQLDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEERPYNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQ
UniProt reference sequence
FDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIREIKILRQLIHRSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFL
Aligned reference sequence
FDII----------------GIIGEGT----------------YGQVYKAKD--KD------------------------------------TGELVALKKVR--LDNEKEGF--------------------------PITAIREIKILRQL--------------------------IHRSVVNMKEIVTDKQ--DALDFKKDKG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFYLVFEYMD---------------HDLMGLLES--GLV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HFSEDHIKSFMKQLMEGLEYCHKK---------------------------------------------NFLHRDI-----KCSNILLNN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGQIKLADFGLARLYNSEESR--PY--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TNKVITLWYRPPELLLG--E-----------------------------ERYT-PAIDVWSCGCILGELFTK-----------------KPIFQA--------------------------------------------------NLELAQLELISRL--CGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIP------------------------------------------------------------------------SAALDLLDHMLTLDPSKRCT--------------AEQTLQSDFL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FDII----------------GIIGEGT----------------YGQVYKAKD--KD------------------------------------TGELVALKKVR--LDNEKEGF--------------------------PITAIREIKILRQL--------------------------IHRSVVNMKEIVTDKQ----LDFKKDKG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFYLVFEYMD---------------HDLMGLLES--GLV------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HFSEDHIKSFMKQLMEGLEYCHKK---------------------------------------------NFLHRDI-----KCSNILLNN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGQIKLADFGLARLYNSEE-R--PY---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKVITLWYRPPELLLG--E-----------------------------ERYT-PAIDVWSCGCILGELFTK-----------------KPIFQA--------------------------------------------------NLELAQLELISRL--CGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIP------------------------------------------------------------------------SAALDLLDHMLTLDPSKRCT--------------AEQTLQSDFL
Activation segment
DFGLARLYNSEE-R--PY---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKVITLWYRPPE
Binding pocket
GIIGEGTYGQVYKVALKKVTAIREIKILRQLIRSVVNMKEIYLVFEYMD_HDLMGLLESYCHKKNFLHRDIKCSNILLLADFGLA
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4CXA, Chain A