4BBE Chain C
Tyrosine-protein kinase JAK2 (JAK2)
Active — 99.9%DFG-inαC-inType1 · 3O4
Resolution
1.9 Å
R-value
0.344
Predicted activity confidence99.9%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
PTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRNLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRD
UniProt reference sequence
LIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNS
Aligned reference sequence
LIFN----------------ESLGQGT----------------FTKIFKGVR--REVGDYGQL-----------------------------HETEVLLKVLD--KAHRNY----------------------------SESFFEAASMMSKL--------------------------SHKHLVLNYGVCVCGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ENILVQEFVKF--------------GSLDTYLKK--NKN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CINILWKLEVAKQLAWAMHFLEEN---------------------------------------------TLIHGNV-----CAKNILLIR--EEDRKTGN----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PPFIKLSDPGISITVLPK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DILQERIPWVPPECIEN--P-----------------------------KNLN-LATDKWSFGTTLWEICSG--G--------------DKPLSA--------------------------------------------------LDSQRKLQFYEDR--HQLPAPKW-------------------------------------------------------------------------------------------------------AELANLINNCMDYEPDFRPS--------------FRAIIRDLNS
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LKFL----------------QQLGKG--------------------NFGSVE--MCRYDPLQD-----------------------------NTGEVVAVKKL--QHSTEE----------------------------HLRDFEREIEILKL--------------------------QHDNIVKYKGVCYSGR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RNLLIMEYLPY--------------GSLRDYLQK--HKE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RIDHIKLLQYTSQICKGMEYLGTK---------------------------------------------RYIHRNL-----ATRNILVEN--ENRVKIGD----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FGLTK---------VLPK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPGESPIFWYAPESLTE--S-----------------------------K-FS-VASDVWSFGVVLYELFTY--I--------------EKSKSP--------------------------------------------------PAEFMRMIGNDKQ--GQMIVFHL-------------------------------------------------------------------------------------------------------IEL--LKNNGRLPRPDQRPS--------------FRDQIRD---
Activation segment
-------VLPK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPGESPIFWYAPE
Binding pocket
QQLGKGNFGSVEMVAVKKLDFEREIEILKSLQDNIVKYKGVKLIMEYLPYGSLRDYLQKYLGTKRYIHRNLATRNILVIGDFGLT
Ligand info
Orthosteric ligand
3O4
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4BBE, Chain C