4AZF Chain A
Dual specificity tyrosine-phosphorylation-regulated kinase 2 (DYRK2)
Active — 95.0%DFG-inαC-inType1 · 3RA
Resolution
2.55 Å
R-value
0.2
Predicted activity confidence95.0%
Structure info
Alternate conformationA
Missing atoms6
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
HHHSGVDLGTENLYFQSMGKVKATPMTPEQAMKQYMQKLTAFEHHEIFSYPEIYFLGLNAKKRQGMTGGPNNGGYDDDQGSYVQVPHDHVAYRYEVLKVIGKGSFGQVVKAYDHKVHQHVALKMVRNEKRFHRQAAEEIRILEHLRKQDKDNTMNVIHMLENFTFRNHICMTFELLSMNLYELIKKNKFQGFSLPLVRKFAHSILQCLDALHKNRIIHCDLKPENILLKQQGRSGIKVIDFGSSCYEHQRVYTIQSRFYRAPEVILGARYGMPIDMWSLGCILAELLTGYPLLPGEDEGDQLACMIELLGMPSQKLLDASKRAKNFVSKGYPRYCTVTTLSDVVLNGGRSRRGKLRGPPESREWGNALKGCDDPLFLDFLKQCLEWDPAVRMTPGQALRHPWLR
UniProt reference sequence
YEVLKVIGKGSFGQVVKAYDHKVHQHVALKMVRNEKRFHRQAAEEIRILEHLRKQDKDNTMNVIHMLENFTFRNHICMTFELLSMNLYELIKKNKFQGFSLPLVRKFAHSILQCLDALHKNRIIHCDLKPENILLKQQGRSGIKVIDFGSSCYEHQRVYTYIQSRFYRAPEVILGARYGMPIDMWSLGCILAELLTGYPLLPGEDEGDQLACMIELLGMPSQKLLDASKRAKNFVSSKGYPRYCTVTTLSDGSVVLNGGRSRRGKLRGPPESREWGNALKGCDDPLFLDFLKQCLEWDPAVRMTPGQALRHPWL
Aligned reference sequence
YEVL----------------KVIGKGS----------------FGQVVKAYD--HK------------------------------------VHQHVALKMVR--NEKRF-----------------------------HRQAAEEIRILEHL--RKQDKD------------------NTMNVIHMLENFTFRN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HICMTFELLS---------------MNLYELIKK--NKFQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFSLPLVRKFAHSILQCLDALHKN---------------------------------------------RIIHCDL-----KPENILLKQ--QG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RSGIKVIDFGSSCYEHQRV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YTYIQSRFYRAPEVILG--------------------------------ARYG-MPIDMWSLGCILAELLTG-----------------YPLLPG--------------------------------------------------EDEGDQLACMIEL--LGMPSQKLLDASKRAKNFVSSKGYPRYCTVTTLSDGSVVLNGGRSRRGKLRGPPESREWGNALKGCDD-------------------------------------------PLFLDFLKQCLEWDPAVRMT--------------PGQALRHPWL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEVL----------------KVIGKGS----------------FGQVVKAYD--HK------------------------------------VHQHVALKMVR--NEKRF-----------------------------HRQAAEEIRILEHL--RKQDKD------------------NTMNVIHMLENFTFRN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HICMTFELLS---------------MNLYELIKK--NKFQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GFSLPLVRKFAHSILQCLDALHKN---------------------------------------------RIIHCDL-----KPENILLKQ--QG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RSGIKVIDFGSSCYEHQRV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YT-IQSRFYRAPEVILG--------------------------------ARYG-MPIDMWSLGCILAELLTG-----------------YPLLPG--------------------------------------------------EDEGDQLACMIEL--LGMPSQKLLDASKRAKNFV-SKGYPRYCTVTTLSD--VVLNGGRSRRGKLRGPPESREWGNALKGCDD-------------------------------------------PLFLDFLKQCLEWDPAVRMT--------------PGQALRHPWL
Activation segment
DFGSSCYEHQRV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YT-IQSRFYRAPE
Binding pocket
KVIGKGSFGQVVKVALKMVQAAEEIRILEHLRMNVIHMLENCMTFELLS_MNLYELIKKALHKNRIIHCDLKPENILLVIDFGSS
Ligand info
Orthosteric ligand
3RA
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4AZF, Chain A