4AOT Chain A
Serine/threonine-protein kinase 10 (STK10)
Inactive — 0.0%DFG-outαC-inType1 · GW8
Resolution
2.33 Å
R-value
0.219
Predicted activity confidence0.0%
Structure info
Alternate conformationB
Missing atoms5
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
SMRKSREYEHVRRDLDPNEVWEIVGELGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYHDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAGTPYWMAPEVVMYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLEHPFVSSITSNKALRELVAEAKAEV
UniProt reference sequence
WEIVGELGDGAFGKVYKAKNKETGALAAAKVIETKSEEELEDYIVEIEILATCDHPYIVKLLGAYYHDGKLWIMIEFCPGGAVDAIMLELDRGLTEPQIQVVCRQMLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKTLQKRDSFIGTPYWMAPEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLEHPFV
Aligned reference sequence
WEIV----------------GELGDGA----------------FGKVYKAKN--KE------------------------------------TGALAAAKVIE--TKSEEE----------------------------LEDYIVEIEILATC--------------------------DHPYIVKLLGAYYHDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLWIMIEFCPG--------------GAVDAIMLE--LDR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GLTEPQIQVVCRQMLEALNFLHSK---------------------------------------------RIIHRDL-----KAGNVLMTL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGDIRLADFGVSAKNLKTLQK--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DSFIGTPYWMAPEVVMC--ETMKD-------------------------TPYD-YKADIWSLGITLIEMAQI-----------------EPPHHE--------------------------------------------------LNPMRVLLKIAKS--DPPTLLTPSKWS---------------------------------------------------------------------------------------------------VEFRDFLKIALDKNPETRPS--------------AAQLLEHPFV
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
WEIV----------------GELGDGA----------------FGKVYKAKN--KE------------------------------------TGALAAAKVIE--TKSEEE----------------------------LEDYIVEIEILATC--------------------------DHPYIVKLLGAYYHDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLWIMIEFCPG--------------GAVDAIMLE--LDR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GLTEPQIQVVCRQMLEALNFLHSK---------------------------------------------RIIHRDL-----KAGNVLMTL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGDIRLADFGVSA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTPYWMAPEVVM-----------------------------------YD-YKADIWSLGITLIEMAQI-----------------EPPHHE--------------------------------------------------LNPMRVLLKIAKS--DPPTLLTPSKWS---------------------------------------------------------------------------------------------------VEFRDFLKIALDKNPETRPS--------------AAQLLEHPFV
Activation segment
DFGVSA------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GTPYWMAPE
Binding pocket
GELGDGAFGKVYKAAAKVIDYIVEIEILATCDPYIVKLLGAWIMIEFCPGGAVDAIMLEFLHSKRIIHRDLKAGNVLMLADFGVS
Ligand info
Orthosteric ligand
GW8
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4AOT, Chain A