4AE6 Chain A
cAMP-dependent protein kinase catalytic subunit alpha (PRKACA)
Active — 99.9%DFG-inαC-in
Resolution
2.1 Å
R-value
0.219
Predicted activity confidence99.9%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SSDVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFVINEKCGKEFSEF
UniProt reference sequence
FERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWF
Aligned reference sequence
FERI----------------KTLGTGS----------------FGRVMLVKH--KE------------------------------------TGNHYAMKILD--KQKVVKLKQ-------------------------IEHTLNEKRILQAV--------------------------NFPFLVKLEFSFKDNS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYMVMEYVPG--------------GEMFSHLRR--IG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSEPHARFYAAQIVLTFEYLHSL---------------------------------------------DLIYRDL-----KPENLLIDQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGYIQVTDFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WTLCGTPEYLAPEIILS--------------------------------KGYN-KAVDWWALGVLIYEMAAG-----------------YPPFFA--------------------------------------------------DQPIQIYEKIVSG--KVRFPSHFS------------------------------------------------------------------------------------------------------SDLKDLLRNLLQVDLTKRFG--NLKNG-------VNDIKNHKWF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FERI----------------KTLGTGS----------------FGRVMLVKH--KE------------------------------------TGNHYAMKILD--KQKVVKLKQ-------------------------IEHTLNEKRILQAV--------------------------NFPFLVKLEFSFKDNS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLYMVMEYVPG--------------GEMFSHLRR--IG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RFSEPHARFYAAQIVLTFEYLHSL---------------------------------------------DLIYRDL-----KPENLLIDQ--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGYIQVTDFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------W-LCGTPEYLAPEIILS--------------------------------KGYN-KAVDWWALGVLIYEMAAG-----------------YPPFFA--------------------------------------------------DQPIQIYEKIVSG--KVRFPSHFS------------------------------------------------------------------------------------------------------SDLKDLLRNLLQVDLTKRFG--NLKNG-------VNDIKNHKWF
Activation segment
DFGFAKRVKGRT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------W-LCGTPEYLAPE
Binding pocket
KTLGTGSFGRVMLYAMKILHTLNEKRILQAVNPFLVKLEFSYMVMEYVPGGEMFSHLRRYLHSLDLIYRDLKPENLLIVTDFGFA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4AE6, Chain A