4A4O Chain A
Serine/threonine-protein kinase PLK1 (PLK1)
Active — 99.6%DFG-inαC-inType1 · 664
Resolution
2.7 Å
R-value
0.23
Predicted activity confidence99.6%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
EIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAP
UniProt reference sequence
YVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFF
Aligned reference sequence
YVRG----------------RFLGKGG----------------FAKCFEISD--AD------------------------------------TKEVFAGKIVP--KSLLLKPHQ-------------------------REKMSMEISIHRSL--------------------------AHQHVVGFHGFFEDND--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FVFVVLELCRR--------------RSLLELHKR--RK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALTEPEARYYLRQIVLGCQYLHRN---------------------------------------------RVIHRDL-----KLGNLFLNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLEVKIGDFGLATKVEYDGER--K---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTLCGTPNYIAPEVLSK--------------------------------KGHS-FEVDVWSIGCIMYTLLVG-----------------KPPFET--------------------------------------------------SCLKETYLRIKKN--EYSIPKHIN------------------------------------------------------------------------------------------------------PVAASLIQKMLQTDPTARPT--------------INELLNDEFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YVRG----------------RFLGKGG----------------FAKCFEISD--AD------------------------------------TKEVFAGKIVP--KSLLLKPHQ-------------------------REKMSMEISIHRSL--------------------------AHQHVVGFHGFFEDND--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FVFVVLELCRR--------------RSLLELHKR--RK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALTEPEARYYLRQIVLGCQYLHRN---------------------------------------------RVIHRDL-----KLGNLFLNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLEVKIGDFGLATKVEYDGER--K---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTLCGTPNYIAPEVLSK--------------------------------KGHS-FEVDVWSIGCIMYTLLVG-----------------KPPFET--------------------------------------------------SCLKETYLRIKKN--EYSIPKHIN------------------------------------------------------------------------------------------------------PVAASLIQKMLQTDPTARPT--------------INELLNDEFF
Activation segment
DFGLATKVEYDGER--K---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTLCGTPNYIAPE
Binding pocket
RFLGKGGFAKCFEFAGKIVKMSMEISIHRSLAQHVVGFHGFFVVLELCRRRSLLELHKRYLHRNRVIHRDLKLGNLFLIGDFGLA
Ligand info
Orthosteric ligand
664
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 4A4O, Chain A