3R30 Chain A
MAP kinase-activated protein kinase 2 (MAPKAPK2)
Inactive — 0.3%DFG-inαC-inType1 · CD2
Resolution
3.2 Å
R-value
0.284
Predicted activity confidence0.3%
Structure info
Alternate conformationB
Missing atoms26
Missing residues1
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
FHVKSGLQIKKNAIIDDYKVTSQVLGLGIGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPEVLDKSCDMWSLGVIMYILLCGYPPFYSGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKED
UniProt reference sequence
YKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWI
Aligned reference sequence
YKVT--S-------------QVLGLGI----------------NGKVLQIFN--KR------------------------------------TQEKFALKMLQ--D---------------------------------CPKARREVELHWRA--S-----------------------QCPHIVRIVDVYENLY--AGRK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLLIVMECLDG--------------GELFSRIQD--RGDQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFTEREASEIMKSIGEAIQYLHSI---------------------------------------------NIAHRDV-----KPENLLYTS--KRP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NAILKLTDFGFAKETTSHNSL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTPCYTPYYVAPEVLGP--------------------------------EKYD-KSCDMWSLGVIMYILLCG-----------------YPPFYS--NHGL--------------------------------------------AISPGMKTRIRMG--QYEFPNPEWSEVS--------------------------------------------------------------------------------------------------EEVKMLIRNLLKTEPTQRMT--------------ITEFMNHPWI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YKVT--S-------------QVLGLGI-----------------GKVLQIFN--KR------------------------------------TQEKFALKMLQ--D---------------------------------CPKARREVELHWRA--S-----------------------QCPHIVRIVDVYENLY--AGRK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLLIVMECLDG--------------GELFSRIQD-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TEREASEIMKSIGEAIQYLHSI---------------------------------------------NIAHRDV-----KPENLLYTS--KRP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NAILKLTDFGFAKETT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PEVL-------------------------------------D-KSCDMWSLGVIMYILLCG-----------------YPPFYS------------------------------------------------------GMKTRIRMG--QYEFPNPEWSEVS--------------------------------------------------------------------------------------------------EEVKMLIRNLLKTEPTQRMT--------------ITEFMNHPWI
Activation segment
DFGFAKETT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PE
Binding pocket
QVLGLGI_GKVLQFALKMLKARREVELHWRASPHIVRIVDVLIVMECLDGGELFSRIQDYLHSINIAHRDVKPENLLYLTDFGFA
Ligand info
Orthosteric ligand
CD2
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3R30, Chain A