3OMV Chain A
RAF proto-oncogene serine/threonine-protein kinase (RAF1)
Inactive — 8.9%DFG-inαC-inType1 · SM5
Resolution
4.0 Å
R-value
0.283
Predicted activity confidence8.9%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
YYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPK
UniProt reference sequence
VMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIEL
Aligned reference sequence
VMLS----------------TRIGSGS----------------FGTVYKGKW-----------------------------------------HGDVAVKILK--VVDPTPEQ--------------------------FQAFRNEVAVLRKT--------------------------RHVNILLFMGYMTKD---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLAIVTQWCEG--------------SSLYKHLHV--QET------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KFQMFQLIDIARQTAQGMDYLHAK---------------------------------------------NIIHRDM-----KSNNIFLHE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GLTVKIGDFGLATVKSRWSGS--QQV-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EQPTGSVLWMAPEVIRM--QDN---------------------------NPFS-FQSDVYSYGIVLYELMTG-----------------ELPYSH--I-----------------------------------------------NNRDQIIFMVGRG--YASPDLSKLYKNCP-------------------------------------------------------------------------------------------------KAMKRLVADCVKKVKEERPL--------------FPQILSSIEL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
VMLS----------------TRIGSGS----------------FGTVYKGKW-----------------------------------------HGDVAVKILK--VVDPTPEQ--------------------------FQAFRNEVAVLRKT--------------------------RHVNILLFMGYMTKD---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLAIVTQWCEG--------------SSLYKHLHV--QET------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KFQMFQLIDIARQTAQGMDYLHAK---------------------------------------------NIIHRDM-----KSNNIFLHE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GLTVKIGDFGLATV---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PTGSVLWMAPEVIRM--QDN---------------------------NPFS-FQSDVYSYGIVLYELMTG-----------------ELPYSH--I-----------------------------------------------NNRDQIIFMVGRG--YASPDLSKLYKNCP-------------------------------------------------------------------------------------------------KAMKRLVADCVKKVKEERPL--------------FPQILSSIEL
Activation segment
DFGLATV---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PTGSVLWMAPE
Binding pocket
TRIGSGSFGTVYKVAVKILAFRNEVAVLRKTRVNILLFMGYAIVTQWCEGSSLYKHLHVYLHAKNIIHRDMKSNNIFLIGDFGLA
Ligand info
Orthosteric ligand
SM5
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3OMV, Chain A