3NUP Chain A
Cyclin-dependent kinase 6 (CDK6)
Inactive — 0.1%DFG-inαC-outType1 · 3NU
Resolution
2.6 Å
R-value
0.238
Predicted activity confidence0.1%
Structure info
Alternate conformation—
Missing atoms0
Missing residues5
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
QYECVAEIGGKVFKARDLKNGGRFVALKRVLSTIREVAVLRHLETFEHPNVVRLFDVCTVKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYF
UniProt reference sequence
YECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTGEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDVIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYSALSHPYF
Aligned reference sequence
YECV----------------AEIGEGA----------------YGKVFKARD--LKN-----------------------------------GGRFVALKRVR--VQTGEEGM--------------------------PLSTIREVAVLRHL--ETF---------------------EHPNVVRLFDVCTVSR--TDRET-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTLVFEHVD---------------QDLTTYLDK--VPEP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GVPTETIKDMMFQLLRGLDFLHSH---------------------------------------------RVVHRDL-----KPQNILVTS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGQIKLADFGLARIYSFQMAL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TSVVVTLWYRAPEVLLQ--------------------------------SSYA-TPVDLWSVGCIFAEMFRR-----------------KPLFRG--------------------------------------------------SSDVDQLGKILDV--IGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDID---------------------------------------------------------------------------ELGKDLLLKCLTFNPAKRIS--------------AYSALSHPYF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YECV----------------AEIG--------------------GKVFKARD--LKN-----------------------------------GGRFVALKRV--------------------------------------LSTIREVAVLRHL--ETF---------------------EHPNVVRLFDVCTV----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTLVFEHVD---------------QDLTTYLDK--VPEP-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GVPTETIKDMMFQLLRGLDFLHSH---------------------------------------------RVVHRDL-----KPQNILVTS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SGQIKLADFGL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VTLWYRAPEVLLQ--------------------------------SSYA-TPVDLWSVGCIFAEMFRR-----------------KPLFRG--------------------------------------------------SSDVDQLGKILDV--IGLPGEEDWPRDVALPRQAFH--SAQPIEKFVTDID---------------------------------------------------------------------------ELGKDLLLKCLTFNPAKRIS--------------AYSALSHPYF
Activation segment
DFGL--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VTLWYRAPE
Binding pocket
AEIG____GKVFKVALKRVSTIREVAVLRHLEPNVVRLFDVTLVFEHVD_QDLTTYLDKFLHSHRVVHRDLKPQNILVLADFGL_
Ligand info
Orthosteric ligand
3NU
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3NUP, Chain A