3MPM Chain A
Tyrosine-protein kinase Lck (LCK)
Inactive — 1.1%DFG-inαC-inType1 · 5LK
Resolution
1.95 Å
R-value
0.172
Predicted activity confidence1.1%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
WEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRNLRAANILVSDTLSCKIADFGLARLIEDNEYTARFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFF
UniProt reference sequence
LKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLED
Aligned reference sequence
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QGSMS-----------------------------PDAFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRDL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGLARLIEDNEYT--AR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGAKFPIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYPG--------------------------------------------------MTNPEVIQNLERG--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QGSMS-----------------------------PDAFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRNL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGLARLIEDNEYT--AR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FPIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYPG--------------------------------------------------MTNPEVIQNLERG--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Activation segment
DFGLARLIEDNEYT--AR------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FPIKWTAPE
Binding pocket
ERLGAGQFGEVWMVAVKSLAFLAEANLMKQLQQRLVRLYAVYIITEYMENGSLVDFLKTFIEERNYIHRNLRAANILVIADFGLA
Ligand info
Orthosteric ligand
5LK
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3MPM, Chain A