3MFU Chain A
Peripheral plasma membrane protein CASK (CASK)
Active — 99.4%DFG-inαC-inATPlike · ANP
Resolution
2.3 Å
R-value
0.182
Predicted activity confidence99.4%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
DVLFEDVYELCEVIGKGAFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPENVLLASKENSAPVKLGDFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERDRYAYKIHLPETVEQLRKFNARRKLKG
UniProt reference sequence
YELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILFILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWL
Aligned reference sequence
YELC----------------EVIGKGP----------------FSVVRRCIN--RE------------------------------------TGQQFAVKIVD--VAKFTSSPGLS-----------------------TEDLKREASICHML--------------------------KHPHIVELLETYSSDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MLYMVFEFMDG--------------ADLCFEIVK--RADAGF---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VYSEAVASHYMRQILEALRYCHDN---------------------------------------------NIIHRDV-----KPHCVLLAS--KEN---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SAPVKLGGFGVAIQLGESGLV--A---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGRVGTPHFMAPEVVKR--------------------------------EPYG-KPVDVWGCGVILFILLSG-----------------CLPFYG---------------------------------------------------TKERLFEGIIKG--KYKMNPRQWSHIS--------------------------------------------------------------------------------------------------ESAKDLVRRMLMLDPAERIT--------------VYEALNHPWL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YELC----------------EVIGKGA----------------FSVVRRCIN--RE------------------------------------TGQQFAVKIVD--VAKFTSSPGLS-----------------------TEDLKREASICHML--------------------------KHPHIVELLETYSSDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MLYMVFEFMDG--------------ADLCFEIVK--RADAGF---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VYSEAVASHYMRQILEALRYCHDN---------------------------------------------NIIHRDV-----KPENVLLAS--KEN---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SAPVKLGDFGVAIQLGESGLV--A---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGRVGTPHFMAPEVVKR--------------------------------EPYG-KPVDVWGCGVILFILLSG-----------------CLPFYG---------------------------------------------------TKERLFEGIIKG--KYKMNPRQWSHIS--------------------------------------------------------------------------------------------------ESAKDLVRRMLMLDPAERIT--------------VYEALNHPWL
Activation segment
DFGVAIQLGESGLV--A---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GGRVGTPHFMAPE
Binding pocket
EVIGKGAFSVVRRFAVKIVDLKREASICHMLKPHIVELLETYMVFEFMDGADLCFEIVKYCHDNNIIHRDVKPENVLLLGDFGVA
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3MFU, Chain A