3IEC Chain D
Serine/threonine-protein kinase MARK2 (MARK2)
Active — 98.9%DFG-inαC-in
Resolution
2.2 Å
R-value
0.206
Predicted activity confidence98.9%
Structure info
Alternate conformation—
Missing atoms0
Missing residues4
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
HIGNYRLLKTIGAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGWMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGY
UniProt reference sequence
YRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWM
Aligned reference sequence
YRLL----------------KTIGKGN----------------FAKVKLARH--IL------------------------------------TGKEVAVKIID--KTQLNSSS--------------------------LQKLFREVRIMKVL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------FIVHRDL-----KAENLLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFGFSNEFTFGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DTFCGSPPYAAPELFQG--------------------------------KKYDGPEVDVWSLGVILYTLVSG-----------------SLPFDG--------------------------------------------------QNLKELRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKFLILNPSKRGT--------------LEQIMKDRWM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YRLL----------------KTIG--------------------AKVKLARH--IL------------------------------------TGKEVAVKIID--KTQLNSSS--------------------------LQKLFREVRIMKVL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------FIVHRDL-----KAENLLLDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFGFSNEFTFGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DTFCGSPPYAAPELFQG--------------------------------KKYDGPEVDVWSLGVILYTLVSG-----------------SLPFDG--------------------------------------------------QNLKELRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKFLILNPSKRGT--------------LEQIMKDRWM
Activation segment
DFGFSNEFTFGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DTFCGSPPYAAPE
Binding pocket
KTIG____AKVKLVAVKIIKLFREVRIMKVLNPNIVKLFEVYLVMEYASGGEVFDYLVAYCHQKFIVHRDLKAENLLLIADFGFS
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3IEC, Chain D