3FHR Chain A
MAP kinase-activated protein kinase 3 (MAPKAPK3)
Inactive — 3.4%DFG-inαC-inType1 · P4O
Resolution
1.9 Å
R-value
0.228
Predicted activity confidence3.4%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
EPKKYAVTDDYQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETEKYDKSCDMWSLGVIMYILLCGFPPFGFPNPEWSEVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQV
UniProt reference sequence
YQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPNPEWSEVSEDAKQLIRLLLKTDPTERLTITQFMNHPWI
Aligned reference sequence
YQLS--K-------------QVLGLGV----------------NGKVLECFH--RR------------------------------------TGQKCALKLLY--D---------------------------------SPKARQEVDHHWQA--S-----------------------GGPHIVCILDVYENMH--HGKR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLLIIMECMEG--------------GELFSRIQE--RGDQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFTEREAAEIMRDIGTAIQFLHSH---------------------------------------------NIAHRDV-----KPENLLYTS--KEK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DAVLKLTDFGFAKETTQNAL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QTPCYTPYYVAPEVLGP--------------------------------EKYD-KSCDMWSLGVIMYILLCG-----------------FPPFYS--NTGQ--------------------------------------------AISPGMKRRIRLG--QYGFPNPEWSEVS--------------------------------------------------------------------------------------------------EDAKQLIRLLLKTDPTERLT--------------ITQFMNHPWI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YQLS--K-------------QVLGLGV----------------NGKVLECFH--RR------------------------------------TGQKCALKLLY--D---------------------------------SPKARQEVDHHWQA--S-----------------------GGPHIVCILDVYENMH--HGKR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLLIIMECMEG--------------GELFSRIQE--R-DQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFTEREAAEIMRDIGTAIQFLHSH---------------------------------------------NIAHRDV-----KPENLLYTS--KEK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DAVLKLTDFGFAKET-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EKYD-KSCDMWSLGVIMYILLCG-----------------FPPF---------------------------------------------------------------------GFPNPEWSEVS--------------------------------------------------------------------------------------------------EDAKQLIRLLLKTDPTERLT--------------ITQFMNHPWI
Activation segment
DFGFAKET-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Binding pocket
QVLGLGVNGKVLECALKLLKARQEVDHHWQASPHIVCILDVLIIMECMEGGELFSRIQEFLHSHNIAHRDVKPENLLYLTDFGFA
Ligand info
Orthosteric ligand
P4O
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3FHR, Chain A