3ENM Chain C
Dual specificity mitogen-activated protein kinase kinase 6 (MAP2K6)
Inactive — 0.2%DFG-outαC-out
Resolution
2.35 Å
R-value
0.215
Predicted activity confidence0.2%
Structure info
Alternate conformationA
Missing atoms0
Missing residues7
Salt bridge (KinCore)Saltbr-na
Sequence info
PDB sequence
VKADDLEPIELGRGAYGVVEKRHVPSGQIAVKRIRATVNSQEQKRLLDLDISRTVDCPFTVTFYGALFREGDVWICELDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKCDFGISGYLVDDVKPYAPERINPELNQSVKSDIWSLGITIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELQHPFFTLHESKGTDVASFVKLILA
UniProt reference sequence
LEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDSVAKTIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFF
Aligned reference sequence
LEPI----------------MELGRGA----------------YGVVEKMRH--VP------------------------------------SGQIMAVKRIR--ATVNSQE---------------------------QKRLLMDLDISMRT--V-----------------------DCPFTVTFYGALFREG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVWICMELMD---------------TSLDKFYKQ--VIDKGQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TIPEDILGKIAVSIVKALEHLHSK--L------------------------------------------SVIHRDV-----KPSNVLINA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LGQVKMCDFGISGYLVDSVAK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TIDAGCKPYMAPERINP--ELNQ--------------------------KGYS-VKSDIWSLGITMIELAIL-----------------RFPYDS--W-----------------------------------------------GTPFQQLKQVVEE--PSPQLPADKFS----------------------------------------------------------------------------------------------------AEFVDFTSQCLKKNSKERPT--------------YPELMQHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LEPI-----------------ELGRGA----------------YGVVEK-RH--VP------------------------------------SGQI-AVKRIR--ATVNSQE---------------------------QKRLL-DLDIS-RT--V-----------------------DCPFTVTFYGALFREG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVWIC-EL-D---------------TSLDKFYKQ--VIDKGQ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TIPEDILGKIAVSIVKALEHLHSK--L------------------------------------------SVIHRDV-----KPSNVLINA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LGQVK-CDFGISGYLVDDV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KPY-APERINP--ELNQ-----------------------------S-VKSDIWSLGIT-IELAIL-----------------RFPYDS--W-----------------------------------------------GTPFQQLKQVVEE--PSPQLPADKFS----------------------------------------------------------------------------------------------------AEFVDFTSQCLKKNSKERPT--------------YPEL-QHPFF
Activation segment
DFGISGYLVDDV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KPY-APE
Binding pocket
_ELGRGAYGVVEK_AVKRIRLL_DLDIS_RTVPFTVTFYGAWIC_EL_D_TSLDKFYKQLHSKLSVIHRDVKPSNVLI_CDFGIS
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationout
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3ENM, Chain C