3E7O Chain B
Mitogen-activated protein kinase 9 (MAPK9)
Inactive — 0.1%DFG-inαC-inType1 · 35F
Resolution
2.14 Å
R-value
0.219
Predicted activity confidence0.1%
Structure info
Alternate conformation—
Missing atoms0
Missing residues5
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
SQFYSVQVADSTFTVLKRYQQLKPIGSGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGSVIFQGTDHIDQWNKVIEQLGTPSAEFMAALQPTVRNYVENRPAYPGIAFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAAPPPQIREHAIEEWKELIYKEVMD
UniProt reference sequence
YQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYI
Aligned reference sequence
YQQL----------------KPIGSGA----------------QGIVCAAFD--TV------------------------------------LGINVAVKKLS--RPFQNQTH--------------------------AKRAYRELVLLKCV--------------------------NHKNIISLLNVFTPQK--TLEEFQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYLVMELMD---------------ANLCQVIHM-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELDHERMSYLLYQMLCGIKHLHSA---------------------------------------------GIIHRDL-----KPSNIVVKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCTLKILDFGLARTACTNFMM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYVVTRYYRAPEVILG--------------------------------MGYK-ENVDIWSVGCIMGELVKG-----------------CVIFQG--------------------------------------------------TDHIDQWNKVIEQ--LGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKT------------------------------------------------------------SQARDLLSKMLVIDPDKRIS--------------VDEALRHPYI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YQQL----------------KPIGS-------------------GIVCAAFD--TV------------------------------------LGINVAVKKLS--RPFQNQTH--------------------------AKRAYRELVLLKCV--------------------------NHKNIISLLNVFTPQK--TLEEFQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYLVMELMD---------------ANLCQVIHM-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELDHERMSYLLYQMLCGIKHLHSA---------------------------------------------GIIHRDL-----KPSNIVVKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCTLKILDFG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TRYYRAPEVILG--------------------------------MGYK-ENVDIWSVGCIMGELVKG-----------------SVIFQG--------------------------------------------------TDHIDQWNKVIEQ--LGTPSAEFMAALQPTVRNYVENRPAYPGIAFEELFPDWIFPSESERDKIKT------------------------------------------------------------SQARDLLSKMLVIDPDKRIS--------------VDEALRHPYI
Activation segment
DFG----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TRYYRAPE
Binding pocket
KPIGSG___IVCAVAVKKLRAYRELVLLKCVNKNIISLLNVYLVMELMD_ANLCQVIHMHLHSAGIIHRDLKPSNIVVILDFG__
Ligand info
Orthosteric ligand
35F
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3E7O, Chain B