3BYU Chain A
Tyrosine-protein kinase Lck (LCK)
Inactive — 0.1%DFG-outαC-inType2 · AM6
Resolution
2.3 Å
R-value
0.232
Predicted activity confidence0.1%
Structure info
Alternate conformation—
Missing atoms0
Missing residues1
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
KPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTAT
UniProt reference sequence
LKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLED
Aligned reference sequence
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QGSMS-----------------------------PDAFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRDL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGLARLIEDNEYT--AR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGAKFPIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYPG--------------------------------------------------MTNPEVIQNLERG--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QGSMS-----------------------------PDAFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRDL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KFPIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYPG--------------------------------------------------MTNPEVIQNLERG--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Activation segment
DFGL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KFPIKWTAPE
Binding pocket
ERLGAGQFGEVWMVAVKSLAFLAEANLMKQLQQRLVRLYAVYIITEYMENGSLVDFLKTFIEERNYIHRDLRAANILVIADFGL_
Ligand info
Orthosteric ligand
AM6
Allosteric ligand
None
Ligand typeType2
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3BYU, Chain A