3A8W Chain A
Protein kinase C iota type (PRKCI)
Active — 99.8%DFG-inαC-inATPlike · ATP
Resolution
2.1 Å
R-value
0.25
Predicted activity confidence99.8%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
MDPLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNEPVQLPDDDDIVRKIDQSEFEGFEYINPL
UniProt reference sequence
FDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSLSVKAASVLKSFLNKDPKERLGCHPQTGFADIQGHPFF
Aligned reference sequence
FDLL----------------RVIGRGS----------------YAKVLLVRL--KK------------------------------------TDRIYAMKVVK--KELVNDDED-------------------------IDWVQTEKHVFEQA--S-----------------------NHPFLVGLHSCFQTES--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLFFVIEYVNG--------------GDLMFHMQR--QR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLPEEHARFYSAEISLALNYLHER---------------------------------------------GIIYRDL-----KLDNVLLDS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGHIKLTDYGMCKEGLRPGDT--T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STFCGTPNYIAPEILRG--------------------------------EDYG-FSVDWWALGVLMFEMMAG-----------------RSPFDI--VGSSDNPDQ---------------------------------------NTEDYLFQVILEK--QIRIPRSLS------------------------------------------------------------------------------------------------------VKAASVLKSFLNKDPKERLG--CHPQTG------FADIQGHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FDLL----------------RVIGRGS----------------YAKVLLVRL--KK------------------------------------TDRIYAMKVVK--KELVN-----------------------------IDWVQTEKHVFEQA--S-----------------------NHPFLVGLHSCFQTES--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLFFVIEYVNG--------------GDLMFHMQR--QR-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLPEEHARFYSAEISLALNYLHER---------------------------------------------GIIYRDL-----KLDNVLLDS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGHIKLTDYGMCKEGLRPGDT--T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------S-FCGTPNYIAPEILRG--------------------------------EDYG-FSVDWWALGVLMFEMMAG-----------------RSPFD----------------------------------------------------TEDYLFQVILEK--QIRIPRSLS------------------------------------------------------------------------------------------------------VKAASVLKSFLNKDPKERLG--CHPQTG------FADIQGHPFF
Activation segment
DYGMCKEGLRPGDT--T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------S-FCGTPNYIAPE
Binding pocket
RVIGRGSYAKVLLYAMKVVWVQTEKHVFEQASPFLVGLHSCFFVIEYVNGGDLMFHMQRYLHERGIIYRDLKLDNVLLLTDYGMC
Ligand info
Orthosteric ligand
ATP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 3A8W, Chain A