2YM7 Chain A
Serine/threonine-protein kinase Chk1 (CHEK1)
Active — 100.0%DFG-inαC-inType1,Type1 · YM7
Resolution
1.81 Å
R-value
0.174
Predicted activity confidence100.0%
Structure info
Alternate conformationA
Missing atoms0
Missing residues5
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
EDWDLVQTLGEVQLAVNRVTEEAVAVKIVNIKKEICINKMLNHENVVKFYGHRREIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKK
UniProt reference sequence
WDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWY
Aligned reference sequence
WDLV----------------QTLGEGA----------------YGEVQLAVN--RV------------------------------------TEEAVAVKIVD--MKRAVDC---------------------------PENIKKEICINKML--------------------------NHENVVKFYGHRREGN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IQYLFLEYCSG--------------GELFDRIEP--DI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GMPEPDAQRFFHQLMAGVVYLHGI---------------------------------------------GITHRDI-----KPENLLLDE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RDNLKISDFGLATVFRYNNRE--RLL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKMCGTLPYVAPELLKR--------------------------------REFHAEPVDVWSCGIVLTAMLAG-----------------ELPWDQ--P-----------------------------------------------SDSCQEYSDWKEK--KTYLNPWKKID----------------------------------------------------------------------------------------------------SAPLALLHKILVENPSARIT--------------IPDIKKDRWY
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
WDLV----------------QTL---------------------GEVQLAVN--RV------------------------------------TEEAVAVKIV---------------------------------------NIKKEICINKML--------------------------NHENVVKFYGHRRE----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------IQYLFLEYCSG--------------GELFDRIEP--DI-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GMPEPDAQRFFHQLMAGVVYLHGI---------------------------------------------GITHRDI-----KPENLLLDE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RDNLKISDFGLATVFRYNNRE--RLL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKMCGTLPYVAPELLKR--------------------------------REFHAEPVDVWSCGIVLTAMLAG-----------------ELPWDQ--P-----------------------------------------------SDSCQEYSDWKEK--KTYLNPWKKID----------------------------------------------------------------------------------------------------SAPLALLHKILVENPSARIT--------------IPDIKKDRWY
Activation segment
DFGLATVFRYNNRE--RLL-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKMCGTLPYVAPE
Binding pocket
QTLGE_____VQLVAVKIVNIKKEICINKMLNENVVKFYGHYLFLEYCSGGELFDRIEPYLHGIGITHRDIKPENLLLISDFGLA
Ligand info
Orthosteric ligand
YM7
Allosteric ligand
YM7
Ligand typeType1,Type1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2YM7, Chain A