2Y7J Chain A
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (PHKG2)
Active — 100.0%DFG-inαC-inType1 · B49
Resolution
2.5 Å
R-value
0.184
Predicted activity confidence100.0%
Structure info
Alternate conformationB
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
ELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFER
UniProt reference sequence
YDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFF
Aligned reference sequence
YDPK----------------DVIGRGV----------------SSVVRRCVH--RA------------------------------------TGHEFAVKIME--VTAERLSPEQLEEV--------------------REATRRETHILRQV--A-----------------------GHPHIITLIDSYESSS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FMFLVFDLMRK--------------GELFDYLTE--KV-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALSEKETRSIMRSLLEAVSFLHAN---------------------------------------------NIVHRDL-----KPENILLDD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NMQIRLSDFGFSCHLEPGEKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RELCGTPGYLAPEILKC--SMDETH------------------------PGYG-KEVDLWACGVILFTLLAG-----------------SPPFWH--------------------------------------------------RRQILMLRMIMEG--QYQFSSPEWDDRS--------------------------------------------------------------------------------------------------STVKDLISRLLQVDPEARLT--------------AEQALQHPFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YDPK----------------DVIGRGV----------------SSVVRRCVH--RA------------------------------------TGHEFAVKIME--VTAERLSPEQLEEV--------------------REATRRETHILRQV--A-----------------------GHPHIITLIDSYESSS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FMFLVFDLMRK--------------GELFDYLTE--KV-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALSEKETRSIMRSLLEAVSFLHAN---------------------------------------------NIVHRDL-----KPENILLDD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NMQIRLSDFGFSCHLEPGEKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RELCGTPGYLAPEILKC--SMDETH------------------------PGYG-KEVDLWACGVILFTLLAG-----------------SPPFWH--------------------------------------------------RRQILMLRMIMEG--QYQFSSPEWDDRS--------------------------------------------------------------------------------------------------STVKDLISRLLQVDPEARLT--------------AEQALQHPFF
Activation segment
DFGFSCHLEPGEKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RELCGTPGYLAPE
Binding pocket
DVIGRGVSSVVRRFAVKIMATRRETHILRQVAPHIITLIDSFLVFDLMRKGELFDYLTEFLHANNIVHRDLKPENILLLSDFGFS
Ligand info
Orthosteric ligand
B49
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2Y7J, Chain A