2XIK Chain A
Serine/threonine-protein kinase 25 (STK25)
Active — 99.8%DFG-inαC-inType1 · J60
Resolution
1.97 Å
R-value
0.191
Predicted activity confidence99.8%
Structure info
Alternate conformationA
Missing atoms8
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
HLRGFANQHSRVDPEELFTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEEIEDIQQEITVLSQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQHSKPFKEFVEACLNKDPRFRPTAKELLKHKFITRYTKKTSFLTELIDRYKRWKS
UniProt reference sequence
FTKLDRIGKGSFGEVYKGIDNHTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQHSKPFKEFVEACLNKDPRFRPTAKELLKHKFI
Aligned reference sequence
FTKL----------------DRIGKGS----------------FGEVYKGID--NH------------------------------------TKEVVAIKIID--LEEAEDE---------------------------IEDIQQEITVLSQC--------------------------DSPYITRYFGSYLKST--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLWIIMEYLGG--------------GSALDLLKP--G--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLEETYIATILREILKGLDYLHSE---------------------------------------------RKIHRDI-----KAANVLLSE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGDVKLADFGVAGQLTDTQIK--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NTFVGTPFWMAPEVIKQ--------------------------------SAYD-FKADIWSLGITAIELAKG-----------------EPPNSD--------------------------------------------------LHPMRVLFLIPKN--SPPTLEGQHS-----------------------------------------------------------------------------------------------------KPFKEFVEACLNKDPRFRPT--------------AKELLKHKFI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FTKL----------------DRIGKGS----------------FGEVYKGID--NH------------------------------------TKEVVAIKIID--LEEAE-E---------------------------IEDIQQEITVLSQC--------------------------DSPYITRYFGSYLKST--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLWIIMEYLGG--------------GSALDLLKP--G--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLEETYIATILREILKGLDYLHSE---------------------------------------------RKIHRDI-----KAANVLLSE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGDVKLADFGVAGQLTDTQIK--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------N-FVGTPFWMAPEVIKQ--------------------------------SAYD-FKADIWSLGITAIELAKG-----------------EPPNSD--------------------------------------------------LHPMRVLFLIPKN--SPPTLEGQHS-----------------------------------------------------------------------------------------------------KPFKEFVEACLNKDPRFRPT--------------AKELLKHKFI
Activation segment
DFGVAGQLTDTQIK--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------N-FVGTPFWMAPE
Binding pocket
DRIGKGSFGEVYKVAIKIIDIQQEITVLSQCDPYITRYFGSWIIMEYLGGGSALDLLKPYLHSERKIHRDIKAANVLLLADFGVA
Ligand info
Orthosteric ligand
J60
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2XIK, Chain A