2XA4 Chain A
Tyrosine-protein kinase JAK2 (JAK2)
Inactive — 0.0%DFG-inαC-inType1 · AZ5
Resolution
2.04 Å
R-value
0.2
Predicted activity confidence0.0%
Structure info
Alternate conformationB
Missing atoms6
Missing residues2
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
QFEERHLKFLQQLGKFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSNLKLIMEYLPYGSLRDYLQAHAERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEKVESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNM
UniProt reference sequence
LIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNS
Aligned reference sequence
LIFN----------------ESLGQGT----------------FTKIFKGVR--REVGDYGQL-----------------------------HETEVLLKVLD--KAHRNY----------------------------SESFFEAASMMSKL--------------------------SHKHLVLNYGVCVCGD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ENILVQEFVKF--------------GSLDTYLKK--NKN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CINILWKLEVAKQLAWAMHFLEEN---------------------------------------------TLIHGNV-----CAKNILLIR--EEDRKTGN----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PPFIKLSDPGISITVLPK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DILQERIPWVPPECIEN--P-----------------------------KNLN-LATDKWSFGTTLWEICSG--G--------------DKPLSA--------------------------------------------------LDSQRKLQFYEDR--HQLPAPKW-------------------------------------------------------------------------------------------------------AELANLINNCMDYEPDFRPS--------------FRAIIRDLNS
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
QFEE----------------RHLKFLQ----------------QLGKFGSVE--MCRYDPLQD-----------------------------NTGEVVAVKKL--QHSTEE----------------------------HLRDFEREIEILKL--------------------------QHDNIVKYKG--VCYS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NLKLIMEYLPY--------------GSLRDYLQA--HAE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RIDHIKLLQYTSQICKGMEYLGTK---------------------------------------------RYIHRDL-----ATRNILVEN--ENRVKIGD----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FGLTK---------VLPK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EKVESPIFWYAPESLTE--S-----------------------------K-FS-VASDVWSFGVVLYELFTY--I--------------EKSKSP--------------------------------------------------PAEFMRMIGNDKQ--GQMIVFHL-------------------------------------------------------------------------------------------------------IEL--LKNNGRLPRPDQRPS--------------FRDQIRD-NM
Activation segment
-------VLPK---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EKVESPIFWYAPE
Binding pocket
QQLGK__FGSVEMVAVKKLDFEREIEILKSLQDNIVKYKGVKLIMEYLPYGSLRDYLQAYLGTKRYIHRDLATRNILVIGDFGLT
Ligand info
Orthosteric ligand
AZ5
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2XA4, Chain A