2X7F Chain E
TRAF2 and NCK-interacting protein kinase (TNIK)
Inactive — 0.0%DFG-inαC-inType1 · 824
Resolution
2.8 Å
R-value
0.21
Predicted activity confidence0.0%
Structure info
Alternate conformationB
Missing atoms135
Missing residues7
Salt bridge (KinCore)Saltbr-none
Sequence info
PDB sequence
DLSALRDPAGIFELVELVGGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKDDQLWLVMEFCGAGSVTDLIKNKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFTPYWMAPEVIDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPEQLMKHPFIRDQPNERQVRIQLKDHIDRT
UniProt reference sequence
FELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKNHSQRPATEQLMKHPFI
Aligned reference sequence
FELV----------------ELVGNGT----------------YGQVYKGRH--VK------------------------------------TGQLAAIKVMD--VTGDE-----------------------------EEEIKQEINMLKKY--S-----------------------HHRNIATYYGAFIKKN--PPGMDD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLWLVMEFCGA--------------GSVTDLIKN--TKGN-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLKEEWIAYICREILRGLSHLHQH---------------------------------------------KVIHRDI-----KGQNVLLTE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NAEVKLVDFGVSAQLDRTVGR--R---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NTFIGTPYWMAPEVIAC--DENPD-------------------------ATYD-FKSDLWSLGITAIEMAEG-----------------APPLCD--------------------------------------------------MHPMRALFLIPRN--PAPRLKSKKWS----------------------------------------------------------------------------------------------------KKFQSFIESCLVKNHSQRPA--------------TEQLMKHPFI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FELV----------------ELVG--------------------GQVYKGRH--VK------------------------------------TGQLAAIKVMD--VTGDE-----------------------------EEEIKQEINMLKKY--S-----------------------HHRNIATYYGAFIKK-------DD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QLWLVMEFCGA--------------GSVTDLIKN-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KEEWIAYICREILRGLSHLHQH---------------------------------------------KVIHRDI-----KGQNVLLTE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NAEVKLVDF-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYWMAPEVI-------------------------------------D-FKSDLWSLGITAIEMAEG-----------------APPLCD--------------------------------------------------MHPMRALFLIPRN--PAPRLKSKKWS----------------------------------------------------------------------------------------------------KKFQSFIESCLVKNHSQRP----------------EQLMKHPFI
Activation segment
DF-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYWMAPE
Binding pocket
ELVG____GQVYKAAIKVMEIKQEINMLKKYSRNIATYYGAWLVMEFCGAGSVTDLIKNHLHQHKVIHRDIKGQNVLLLVDF___
Ligand info
Orthosteric ligand
824
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2X7F, Chain E