2WTK Chain B
STE20-related kinase adapter protein alpha (STRADA)
Inactive — 34.3%DFG-outαC-inATPlike · ANP
Resolution
2.65 Å
R-value
0.24
Predicted activity confidence34.3%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-none
Sequence info
PDB sequence
SFLPEGGCYELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTFLQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQGVLKALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKVLPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGHVPFDMPATQMLLEKLVPCLFSPHFHHFVEQCLQRNPDARPSASTLLNHSFFKQIKRRASEALPELLRPVTPITNFEGSQSQDHSGIFGLVTDWEF
UniProt reference sequence
YELLTVIGKGFEDLMTVNLARYKPTGEYVTVRRINLEACSNEMVTFLQGELHVSKLFNHPNIVPYRATFIADNELWVVTSFMAYGSAKDLICTHFMDGMNELAIAYILQGVLKALDYIHHMGYVHRSVKASHILISVDGKVYLSGLRSNLSMISHGQRQRVVHDFPKYSVKVLPWLSPEVLQQNLQGYDAKSDIYSVGITACELANGHVPFKDMPATQMLLEKLNGTVPCLLDTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRPSNGDSPSHPYHRTFSPHFHHFVEQCLQRNPDARPSASTLLNHSFF
Aligned reference sequence
YELL----------------TVIGKGF--ED------------LMTVNLARY--KP------------------------------------TGEYVTVRRIN--LEACSNEM--------------------------VTFLQGELHVSKLF--------------------------NHPNIVPYRATFIADN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWVVTSFMAY--------------GSAKDLICT--HFMD-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GMNELAIAYILQGVLKALDYIHHM---------------------------------------------GYVHRSV-----KASHILISV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGKVYLSGLRSNLSMISHGQR--QRVVHDFP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KYSVKVLPWLSPEVLQQ--NL----------------------------QGYD-AKSDIYSVGITACELANG-----------------HVPFKD--------------------------------------------------MPATQMLLEKLNG--TVPCLLDTSTIPAEELTMSPSRSVANSGLSDSLTTSTPRPSNGDSPSHPYHRTFS--------------------------------------------------------PHFHHFVEQCLQRNPDARPS--------------ASTLLNHSFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YELL----------------TVIGKGF--ED------------LMTVNLARY--KP------------------------------------TGEYVTVRRIN--LEACSNEM--------------------------VTFLQGELHVSKLF--------------------------NHPNIVPYRATFIADN--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELWVVTSFMAY--------------GSAKDLICT--HFMD-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GMNELAIAYILQGVLKALDYIHHM---------------------------------------------GYVHRSV-----KASHILISV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DGKVYLSGLRSNLSMISHGQR--QRVVHDFP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KYSVKVLPWLSPEVLQQ--NL----------------------------QGYD-AKSDIYSVGITACELANG-----------------HVPF-D--------------------------------------------------MPATQMLLEKL-----VPCL------------------------------------------------FS--------------------------------------------------------PHFHHFVEQCLQRNPDARPS--------------ASTLLNHSFF
Activation segment
GLRSNLSMISHGQR--QRVVHDFP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KYSVKVLPWLSPE
Binding pocket
TVIGKGFEDLMTVVTVRRIFLQGELHVSKLFNPNIVPYRATWVVTSFMAYGSAKDLICTYIHHMGYVHRSVKASHILILSGLRSN
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2WTK, Chain B