2W4K Chain A
Death-associated protein kinase 1 (DAPK1)
Active — 100.0%DFG-inαC-inATPlike · ADP
Resolution
1.9 Å
R-value
0.195
Predicted activity confidence100.0%
Structure info
Alternate conformationA
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
TVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFAAR
UniProt reference sequence
YDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSREDIEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFLKQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGTPEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEYFSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWI
Aligned reference sequence
YDTG----------------EELGSGQ----------------FAVVKKCRE--KS------------------------------------TGLQYAAKFIK--KRRTKSSRRGVS----------------------REDIEREVSILKEI--------------------------QHPNVITLHEVYENKT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVILILELVAG--------------GELFDFLAE--KE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLTEEEATEFLKQILNGVYYLHSL---------------------------------------------QIAHFDL-----KPENIMLLD--RNVP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KPRIKIIDFGLAHKIDFGNEF------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KNIFGTPEFVAPEIVNY--------------------------------EPLG-LEADMWSIGVITYILLSG-----------------ASPFLG--------------------------------------------------DTKQETLANVSAV--NYEFEDEYFSNTS--------------------------------------------------------------------------------------------------ALAKDFIRRLLVKDPKKRMT--------------IQDSLQHPWI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YDTG----------------EELGSGQ----------------FAVVKKCRE--KS------------------------------------TGLQYAAKFIK--KRRTKSSRRGVS----------------------REDIEREVSILKEI--------------------------QHPNVITLHEVYENKT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVILILELVAG--------------GELFDFLAE--KE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SLTEEEATEFLKQILNGVYYLHSL---------------------------------------------QIAHFDL-----KPENIMLLD--RNVP--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KPRIKIIDFGLAHKIDFGNEF------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KNIFGTPEFVAPEIVNY--------------------------------EPLG-LEADMWSIGVITYILLSG-----------------ASPFLG--------------------------------------------------DTKQETLANVSAV--NYEFEDEYFSNTS--------------------------------------------------------------------------------------------------ALAKDFIRRLLVKDPKKRMT--------------IQDSLQHPWI
Activation segment
DFGLAHKIDFGNEF------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KNIFGTPEFVAPE
Binding pocket
EELGSGQFAVVKKYAAKFIDIEREVSILKEIQPNVITLHEVILILELVAGGELFDFLAEYLHSLQIAHFDLKPENIMLIIDFGLA
Ligand info
Orthosteric ligand
ADP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2W4K, Chain A