2V5Q Chain B
Serine/threonine-protein kinase PLK1 (PLK1)
Active — 94.7%DFG-inαC-in
Resolution
2.3 Å
R-value
0.184
Predicted activity confidence94.7%
Structure info
Alternate conformationA
Missing atoms2
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
EIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPP
UniProt reference sequence
YVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFF
Aligned reference sequence
YVRG----------------RFLGKGG----------------FAKCFEISD--AD------------------------------------TKEVFAGKIVP--KSLLLKPHQ-------------------------REKMSMEISIHRSL--------------------------AHQHVVGFHGFFEDND--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FVFVVLELCRR--------------RSLLELHKR--RK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALTEPEARYYLRQIVLGCQYLHRN---------------------------------------------RVIHRDL-----KLGNLFLNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLEVKIGDFGLATKVEYDGER--K---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTLCGTPNYIAPEVLSK--------------------------------KGHS-FEVDVWSIGCIMYTLLVG-----------------KPPFET--------------------------------------------------SCLKETYLRIKKN--EYSIPKHIN------------------------------------------------------------------------------------------------------PVAASLIQKMLQTDPTARPT--------------INELLNDEFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YVRG----------------RFLGKGG----------------FAKCFEISD--AD------------------------------------TKEVFAGKIVP--KSLLLKPHQ-------------------------REKMSMEISIHRSL--------------------------AHQHVVGFHGFFEDND--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FVFVVLELCRR--------------RSLLELHKR--RK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ALTEPEARYYLRQIVLGCQYLHRN---------------------------------------------RVIHRDL-----KLGNLFLNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLEVKIGDFGLATKVEYDGER--K---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTLCGTPNYIAPEVLSK--------------------------------KGHS-FEVDVWSIGCIMYTLLVG-----------------KPPFET--------------------------------------------------SCLKETYLRIKKN--EYSIPKHIN------------------------------------------------------------------------------------------------------PVAASLIQKMLQTDPTARPT--------------INELLNDEFF
Activation segment
DFGLATKVEYDGER--K---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTLCGTPNYIAPE
Binding pocket
RFLGKGGFAKCFEFAGKIVKMSMEISIHRSLAQHVVGFHGFFVVLELCRRRSLLELHKRYLHRNRVIHRDLKLGNLFLIGDFGLA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2V5Q, Chain B