2R5T Chain A
Serine/threonine-protein kinase Sgk1 (SGK1)
Inactive — 0.2%DFG-inαC-outATPlike · ANP
Resolution
1.9 Å
R-value
0.193
Predicted activity confidence0.2%
Structure info
Alternate conformation—
Missing atoms2
Missing residues7
Salt bridge (KinCore)Saltbr-none
Sequence info
PDB sequence
QINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSG
UniProt reference sequence
FHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFF
Aligned reference sequence
FHFL----------------KVIGKGS----------------FGKVLLARH--KA------------------------------------EEVFYAVKVLQ--KKAILKKKE-------------------------EKHIMSERNVLLKN--V-----------------------KHPFLVGLHFSFQTAD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLYFVLDYING--------------GELFYHLQR--ER-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CFLEPRARFYAAEIASALGYLHSL---------------------------------------------NIVYRDL-----KPENILLDS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGHIVLTDFGLCKENIEHNST--T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STFCGTPEYLAPEVLHK--------------------------------QPYD-RTVDWWCLGAVLYEMLYG-----------------LPPFYS--------------------------------------------------RNTAEMYDNILNK--PLQLKPNIT------------------------------------------------------------------------------------------------------NSARHLLEGLLQKDRTKRLG--AKDD--------FMEIKSHVFF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
FHFL----------------KVIGKGS----------------FGKVLLARH--KA------------------------------------EEVFYAVKVLQ--KKAIL--------------------------------------VLLKN--V-----------------------KHPFLVGLHFSFQTAD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLYFVLDYING--------------GELFYHLQR--ER-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CFLEPRARFYAAEIASALGYLHSL---------------------------------------------NIVYRDL-----KPENILLDS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QGHIVLTDFGLCKENIEHNST--T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STFCGTPEYLAPEVLHK--------------------------------QPYD-RTVDWWCLGAVLYEMLYG-----------------LPPFYS--------------------------------------------------RNTAEMYDNILNK--PLQLKPNIT------------------------------------------------------------------------------------------------------NSARHLLEGLLQKDRTKRLG--AKDD--------FMEIKSHVFF
Activation segment
DFGLCKENIEHNST--T---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------STFCGTPEYLAPE
Binding pocket
KVIGKGSFGKVLLYAVKVL_______VLLKNVPFLVGLHFSYFVLDYINGGELFYHLQRYLHSLNIVYRDLKPENILLLTDFGLC
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2R5T, Chain A