2OFV Chain B
Tyrosine-protein kinase Lck (LCK)
Inactive — 0.0%DFG-outαC-inType2 · 242
Resolution
2.0 Å
R-value
0.274
Predicted activity confidence0.0%
Structure info
Alternate conformation—
Missing atoms10
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
PWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPIQNLEGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFT
UniProt reference sequence
LKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLED
Aligned reference sequence
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QGSMS-----------------------------PDAFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRDL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGLARLIEDNEYT--AR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGAKFPIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYPG--------------------------------------------------MTNPEVIQNLERG--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QG----------------------------------AFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRDL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGLARLIED---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYP---------------------------------------------------------IQNLE-G--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Activation segment
DFGLARLIED---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIKWTAPE
Binding pocket
ERLGAGQFGEVWMVAVKSLAFLAEANLMKQLQQRLVRLYAVYIITEYMENGSLVDFLKTFIEERNYIHRDLRAANILVIADFGLA
Ligand info
Orthosteric ligand
242
Allosteric ligand
None
Ligand typeType2
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2OFV, Chain B