2JII Chain B
Serine/threonine-protein kinase VRK3 (VRK3)
Active — 97.5%DFG-inαC-out
Resolution
2.0 Å
R-value
0.181
Predicted activity confidence97.5%
Structure info
Alternate conformationB
Missing atoms4
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
TENLYFQSMTTSLEALPTGTVLTDKSGRQWKLKSFQTRDNQGILYEAAPTSQKFSLKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSTPLLAIPTCMGFGVHQDKYRFLVLPSLGRSLQSALDVSPKHVLSERSVLQVACRLLDALEFLHENEYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCPSGKHVAYVEGSRSPHEGDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIMKQKQKFVDKPGPFVGPCGHWIRPSETLQKYLKVVMALTYEEKPPYAMLRNNLEALLQDLRVSPYDPIGLPM
UniProt reference sequence
WKLKSFQTRDNQGILYEAAPTSTLTCDSGPQKQKFSLKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSTPLLAIPTCMGFGVHQDKYRFLVLPSLGRSLQSALDVSPKHVLSERSVLQVACRLLDALEFLHENEYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCPSGKHVAYVEGSRSPHEGDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIMKQKQKFVDKPGPFVGPCGHWIRPSETLQKYLKVVMALTYEEKPPYAMLRNNLEA
Aligned reference sequence
WKLK----------------SFQTRDN----------------QGILYEAAP--TSTLTCDSGP----------------------------QKQKFSLKLDA--K---------------------------------DGRLFNEQNFFQRA--AKPLQVNKWKKLYSTPL---------LAIPTCMGFGVHQD--K-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YRFLVLPSLG---------------RSLQSALDV--SPKH-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLSERSVLQVACRLLDALEFLHEN---------------------------------------------EYVHGNV-----TAENIFVDP--ED----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QSQVTLAGYGFAFRYCPSGKH--VAYVEGSR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SPHEGDLEFISMDLHKG--------------------------------CGPS-RRSDLQSLGYCMLKWLYG-----------------FLPWTN--CL----------------------------------------------PNTEDIMKQKQKF--VDKPGPFVGPCGHWIRPS---------------------------------------------------------------------------------------------ETLQKYLKVVMALTYEEKPP--------------YAMLRNNLEA
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
WKLK----------------SFQTRDN----------------QGILYEAAP--TS--------------------------------------QKFSLKLDA--K---------------------------------DGRLFNEQNFFQRA--AKPLQVNKWKKLYSTPL---------LAIPTCMGFGVHQD--K-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YRFLVLPSLG---------------RSLQSALDV--SPKH-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VLSERSVLQVACRLLDALEFLHEN---------------------------------------------EYVHGNV-----TAENIFVDP--ED----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QSQVTLAGYGFAFRYCPSGKH--VAYVEGSR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SPHEGDLEFISMDLHKG--------------------------------CGPS-RRSDLQSLGYCMLKWLYG-----------------FLPWTN--CL----------------------------------------------PNTEDIMKQKQKF--VDKPGPFVGPCGHWIRPS---------------------------------------------------------------------------------------------ETLQKYLKVVMALTYEEKPP--------------YAMLRNNLEA
Activation segment
GYGFAFRYCPSGKH--VAYVEGSR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SPHEGDLEFISMD
Binding pocket
SFQTRDNQGILYEFSLKLDLQVNKWKKLYSTPLAIPTCMGFFLVLPSLG_RSLQSALDVFLHENEYVHGNVTAENIFVLAGYGFA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationout
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2JII, Chain B