2HAK Chain B
Serine/threonine-protein kinase MARK1 (MARK1)
Inactive — 0.2%DFG-inαC-in
Resolution
2.6 Å
R-value
0.214
Predicted activity confidence0.2%
Structure info
Alternate conformation—
Missing atoms12
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
EQPHIGNYRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVDVWSLGVILYTLVSGSLPFDGLRERVLRGKYRIPFYMSTDCENLLKKLLVLNPRGSLEQIMKDRWMNVGELKPYTEPDPDFNDTKRIDIMVTMGFARDEINDALINQKYDEVMATYILLGRK
UniProt reference sequence
YRLQKTIGKGNFAKVKLARHVLTGREVAVKIIDKTQLNPTSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKYIVHRDLKAENLLLDGDMNIKIADFGFSNEFTVGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKLLVLNPIKRGSLEQIMKDRWM
Aligned reference sequence
YRLQ----------------KTIGKGN----------------FAKVKLARH--VL------------------------------------TGREVAVKIID--KTQLNPTS--------------------------LQKLFREVRIMKIL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------YIVHRDL-----KAENLLLDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFGFSNEFTVGNKL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DTFCGSPPYAAPELFQG--------------------------------KKYDGPEVDVWSLGVILYTLVSG-----------------SLPFDG--------------------------------------------------QNLKELRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKLLVLNPIKRGS--------------LEQIMKDRWM
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YRLQ----------------KTIGKGN----------------FAKVKLARH--VL------------------------------------TGREVAVKIID--KTQLNPTS--------------------------LQKLFREVRIMKIL--------------------------NHPNIVKLFEVIETEK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLYLVMEYASG--------------GEVFDYLVA--HG-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RMKEKEARAKFRQIVSAVQYCHQK---------------------------------------------YIVHRDL-----KAENLLLDG--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DMNIKIADFGFSNEFT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VDVWSLGVILYTLVSG-----------------SLPFDG-------------------------------------------------------LRERVLRG--KYRIPFYMS------------------------------------------------------------------------------------------------------TDCENLLKKLLVLNP--RGS--------------LEQIMKDRWM
Activation segment
DFGFSNEFT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Binding pocket
KTIGKGNFAKVKLVAVKIIKLFREVRIMKILNPNIVKLFEVYLVMEYASGGEVFDYLVAYCHQKYIVHRDLKAENLLLIADFGFS
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2HAK, Chain B