2GMX Chain A
Mitogen-activated protein kinase 8 (MAPK8)
Active — 84.4%DFG-inαC-inType1 · 877
Resolution
3.5 Å
R-value
0.278
Predicted activity confidence84.4%
Structure info
Alternate conformation—
Missing atoms4
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
NNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMEPEVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEH
UniProt reference sequence
YQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYI
Aligned reference sequence
YQNL----------------KPIGSGA----------------QGIVCAAYD--AI------------------------------------LERNVAIKKLS--RPFQNQTH--------------------------AKRAYRELVLMKCV--------------------------NHKNIIGLLNVFTPQK--SLEEFQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVMELMD---------------ANLCQVIQM-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELDHERMSYLLYQMLCGIKHLHSA---------------------------------------------GIIHRDL-----KPSNIVVKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCTLKILDFGLARTAGTSFMM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYVVTRYYRAPEVILG--------------------------------MGYK-ENVDLWSVGCIMGEMVCH-----------------KILFPG--------------------------------------------------RDYIDQWNKVIEQ--LGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKA------------------------------------------------------------SQARDLLSKMLVIDASKRIS--------------VDEALQHPYI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YQNL----------------KPIGSGA----------------QGIVCAAYD--AI------------------------------------LERNVAIKKLS--RPFQNQTH--------------------------AKRAYRELVLMKCV--------------------------NHKNIIGLLNVFTPQK--SLEEFQ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYIVMELMD---------------ANLCQVIQM-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ELDHERMSYLLYQMLCGIKHLHSA---------------------------------------------GIIHRDL-----KPSNIVVKS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCTLKILDFGLARTAGTSFMM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPEVVTRYYRAPEVILG--------------------------------MGYK-ENVDLWSVGCIMGEMVCH-----------------KILFPG--------------------------------------------------RDYIDQWNKVIEQ--LGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKA------------------------------------------------------------SQARDLLSKMLVIDASKRIS--------------VDEALQHPYI
Activation segment
DFGLARTAGTSFMM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EPEVVTRYYRAPE
Binding pocket
KPIGSGAQGIVCAVAIKKLRAYRELVLMKCVNKNIIGLLNVYIVMELMD_ANLCQVIQMHLHSAGIIHRDLKPSNIVVILDFGLA
Ligand info
Orthosteric ligand
877
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2GMX, Chain A