2CN5 Chain A
Serine/threonine-protein kinase Chk2 (CHEK2)
Inactive — 43.7%DFG-inαC-inATPlike · ADP
Resolution
2.25 Å
R-value
0.201
Predicted activity confidence43.7%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-out
Sequence info
PDB sequence
HMSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENE
UniProt reference sequence
YIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEVWAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWL
Aligned reference sequence
YIMS----------------KTLGSGA----------------CGEVKLAFE--RK------------------------------------TCKKVAIKIIS--KRKFAIGSAREADP--------------------ALNVETEIEILKKL--------------------------NHPCIIKIKNFFDAE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYYIVLELMEG--------------GELFDKVVG--NK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLKEATCKLYFYQMLLAVQYLHEN---------------------------------------------GIIHRDL-----KPENVLLSS--QEE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCLIKITDFGHSKILGETSLM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RTLCGTPTYLAPEVLVS--VGT---------------------------AGYN-RAVDCWSLGVILFICLSG-----------------YPPFSE--H-----------------------------------------------RTQVSLKDQITSG--KYNFIPEVWAEVS--------------------------------------------------------------------------------------------------EKALDLVKKLLVVDPKARFT--------------TEEALRHPWL
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YIMS----------------KTLGSGA----------------CGEVKLAFE--RK------------------------------------TCKKVAIKIIS--KR----------------------------------NVETEIEILKKL--------------------------NHPCIIKIKNFFDAE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DYYIVLELMEG--------------GELFDKVVG--NK-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLKEATCKLYFYQMLLAVQYLHEN---------------------------------------------GIIHRDL-----KPENVLLSS--QEE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCLIKITDFGHSKILGETSLM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RTLCGTPTYLAPEVLVS--VGT---------------------------AGYN-RAVDCWSLGVILFICLSG-----------------YPPFSE--H-----------------------------------------------RTQVSLKDQITSG--KYNFIPEVWAEVS--------------------------------------------------------------------------------------------------EKALDLVKKLLVVDPKARFT--------------TEEALRHPWL
Activation segment
DFGHSKILGETSLM------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RTLCGTPTYLAPE
Binding pocket
KTLGSGACGEVKLVAIKIINVETEIEILKKLNPCIIKIKNFYIVLELMEGGELFDKVVGYLHENGIIHRDLKPENVLLITDFGHS
Ligand info
Orthosteric ligand
ADP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2CN5, Chain A