2BAQ Chain A
Mitogen-activated protein kinase 14 (MAPK14)
Inactive — 0.1%DFG-outαC-inType1 · PQB
Resolution
2.8 Å
R-value
0.222
Predicted activity confidence0.1%
Structure info
Alternate conformation—
Missing atoms0
Missing residues6
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
RPTFYRQELNKTIWEVPERYQNLSPIGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDELKILDFGLAYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPP
UniProt reference sequence
YQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYF
Aligned reference sequence
YQNL----------------SPVGSGA----------------YGSVCAAFD--TK------------------------------------TGLRVAVKKLS--RPFQSIIH--------------------------AKRTYRELRLLKHM--------------------------KHENVIGLLDVFTPAR--SLEEFN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYLVTHLMG---------------ADLNNIVKC--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSA---------------------------------------------DIIHRDL-----KPSNLAVNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCELKILDFGLARHTDDEM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGYVATRWYRAPEIMLN--W-----------------------------MHYN-QTVDIWSVGCIMAELLTG-----------------RTLFPG--------------------------------------------------TDHIDQLKLILRL--VGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGAN-----------------------------------------------------------------------PLAVDLLEKMLVLDSDKRIT--------------AAQALAHAYF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YQNL----------------SPIGSGA----------------YGSVCAAFD--TK------------------------------------TGLRVAVKKLS--RPFQSIIH--------------------------AKRTYRELRLLKHM--------------------------KHENVIGLLDVFTPAR--SLEEFN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYLVTHLMG---------------ADL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LTDDHVQFLIYQILRGLKYIHSA---------------------------------------------DIIHRDL-----KPSNLAVNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------D-ELKILDFGLA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YVATRWYRAPEIMLN--W-----------------------------MHYN-QTVDIWSVGCIMAELLTG-----------------RTLFPG--------------------------------------------------TDHIDQLKLILRL--VGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGAN-----------------------------------------------------------------------PLAVDLLEKMLVLDSDKRIT--------------AAQALAHAYF
Activation segment
DFGLA-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YVATRWYRAPE
Binding pocket
SPIGSGAYGSVCAVAVKKLRTYRELRLLKHMKENVIGLLDVYLVTHLMG_ADL______YIHSADIIHRDLKPSNLAVILDFGLA
Ligand info
Orthosteric ligand
PQB
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationout
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 2BAQ, Chain A