1QPJ Chain A
Tyrosine-protein kinase Lck (LCK)
Active — 99.7%DFG-inαC-inType1 · STU
Resolution
2.2 Å
R-value
0.214
Predicted activity confidence99.7%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
DEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNETAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTAT
UniProt reference sequence
LKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLED
Aligned reference sequence
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QGSMS-----------------------------PDAFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRDL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGLARLIEDNEYT--AR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGAKFPIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYPG--------------------------------------------------MTNPEVIQNLERG--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
LKLV----------------ERLGAGQ----------------FGEVWMGYY--N-------------------------------------GHTKVAVKSLK--QGSMS-----------------------------PDAFLAEANLMKQL--------------------------QHQRLVRLYAVVTQE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PIYIITEYMEN--------------GSLVDFLKT--PSGI-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTINKLLDMAAQIAEGMAFIEER---------------------------------------------NYIHRDL-----RAANILVSD--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TLSCKIADFGLARLIEDNE-T--AR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGAKFPIKWTAPEAINY--------------------------------GTFT-IKSDVWSFGILLTEIVTH--G--------------RIPYPG--------------------------------------------------MTNPEVIQNLERG--YRMVRPDNCP-----------------------------------------------------------------------------------------------------EELYQLMRLCWKERPEDRPT--------------FDYLRSVLED
Activation segment
DFGLARLIEDNE-T--AR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------EGAKFPIKWTAPE
Binding pocket
ERLGAGQFGEVWMVAVKSLAFLAEANLMKQLQQRLVRLYAVYIITEYMENGSLVDFLKTFIEERNYIHRDLRAANILVIADFGLA
Ligand info
Orthosteric ligand
STU
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 1QPJ, Chain A