1NXK Chain C
MAP kinase-activated protein kinase 2 (MAPKAPK2)
Inactive — 0.1%DFG-inαC-inType1 · STU
Resolution
2.7 Å
R-value
0.241
Predicted activity confidence0.1%
Structure info
Alternate conformation—
Missing atoms8
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
QQFPQFHVKSGLQIKKNAIIDDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVECLDGGELFSRIQDRGDQAFTEREASEIKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTPYYVAPEVLGPEKYDKSCDWSLGVIYILLCGYPPFYSNHGLAISPGKTRIRGQYEFPNPEWSEVSEEVKLIRNLLKTEPTQRTITEFNHPWIQSTKVPQTPLHTSRVLKEDKERWEDVKEET
UniProt reference sequence
YKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRIVDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSINIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKMLIRNLLKTEPTQRMTITEFMNHPWI
Aligned reference sequence
YKVT--S-------------QVLGLGI----------------NGKVLQIFN--KR------------------------------------TQEKFALKMLQ--D---------------------------------CPKARREVELHWRA--S-----------------------QCPHIVRIVDVYENLY--AGRK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLLIVMECLDG--------------GELFSRIQD--RGDQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFTEREASEIMKSIGEAIQYLHSI---------------------------------------------NIAHRDV-----KPENLLYTS--KRP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NAILKLTDFGFAKETTSHNSL------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTPCYTPYYVAPEVLGP--------------------------------EKYD-KSCDMWSLGVIMYILLCG-----------------YPPFYS--NHGL--------------------------------------------AISPGMKTRIRMG--QYEFPNPEWSEVS--------------------------------------------------------------------------------------------------EEVKMLIRNLLKTEPTQRMT--------------ITEFMNHPWI
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YKVT--S-------------QVLGLGI----------------NGKVLQIFN--KR------------------------------------TQEKFALK-LQ--D---------------------------------CPKARREVELHWRA--S-----------------------QCPHIVRIVDVYENLY--AGRK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CLLIV-ECLDG--------------GELFSRIQD--RGDQ-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------AFTEREASEI-KSIGEAIQYLHSI---------------------------------------------NIAHRDV-----KPENLLYTS--KRP---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NAILKLTDFGFAKET-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYYVAPEVLGP--------------------------------EKYD-KSCD-WSLGVI-YILLCG-----------------YPPFYS--NHGL--------------------------------------------AISPG-KTRIR-G--QYEFPNPEWSEVS--------------------------------------------------------------------------------------------------EEVK-LIRNLLKTEPTQR-T--------------ITEF-NHPWI
Activation segment
DFGFAKET-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TPYYVAPE
Binding pocket
QVLGLGINGKVLQFALKMLKARREVELHWRASPHIVRIVDVLIVMECLDGGELFSRIQDYLHSINIAHRDVKPENLLYLTDFGFA
Ligand info
Orthosteric ligand
STU
Allosteric ligand
None
Ligand typeType1
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 1NXK, Chain C