1LEW Chain A
Mitogen-activated protein kinase 14 (MAPK14)
Inactive — 0.0%DFG-inαC-in
Resolution
2.3 Å
R-value
0.245
Predicted activity confidence0.0%
Structure info
Alternate conformation—
Missing atoms0
Missing residues0
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
RPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGHRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLAQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLD
UniProt reference sequence
YQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYF
Aligned reference sequence
YQNL----------------SPVGSGA----------------YGSVCAAFD--TK------------------------------------TGLRVAVKKLS--RPFQSIIH--------------------------AKRTYRELRLLKHM--------------------------KHENVIGLLDVFTPAR--SLEEFN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYLVTHLMG---------------ADLNNIVKC--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSA---------------------------------------------DIIHRDL-----KPSNLAVNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCELKILDFGLARHTDDEM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGYVATRWYRAPEIMLN--W-----------------------------MHYN-QTVDIWSVGCIMAELLTG-----------------RTLFPG--------------------------------------------------TDHIDQLKLILRL--VGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGAN-----------------------------------------------------------------------PLAVDLLEKMLVLDSDKRIT--------------AAQALAHAYF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YQNL----------------SPVGSGA----------------YGSVCAAFD--TK------------------------------------TGHRVAVKKLS--RPFQSIIH--------------------------AKRTYRELRLLKHM--------------------------KHENVIGLLDVFTPAR--SLEEFN------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DVYLVTHLMG---------------ADLNNIVKC--Q--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTDDHVQFLIYQILRGLKYIHSA---------------------------------------------DIIHRDL-----KPSNLAVNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCELKILDFGLAR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VATRWYRAPEIMLN--W-----------------------------MHYN-QTVDIWSVGCIMAELLTG-----------------RTLFPG--------------------------------------------------TDHIDQLKLILRL--VGTPGAELLKKISSESARNYIQSLAQMPKMNFANVFIGAN-----------------------------------------------------------------------PLAVDLLEKMLVLDSDKRIT--------------AAQALAHAYF
Activation segment
DFGLAR-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VATRWYRAPE
Binding pocket
SPVGSGAYGSVCAVAVKKLRTYRELRLLKHMKENVIGLLDVYLVTHLMG_ADLNNIVKCYIHSADIIHRDLKPSNLAVILDFGLA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityinactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 1LEW, Chain A