1H4L Chain B
Cyclin-dependent kinase 5 (CDK5)
Active — 92.1%DFG-inαC-in
Resolution
2.65 Å
R-value
0.236
Predicted activity confidence92.1%
Structure info
Alternate conformation—
Missing atoms0
Missing residues4
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
QKYEKLEKIYGTVFKAKNRETHEIVALKRVRLDGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAARPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYFS
UniProt reference sequence
YEKLEKIGEGTYGTVFKAKNRETHEIVALKRVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFCDQDLKKYFDSCNGDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLLINRNGELKLADFGLARAFGIPVRCYSAEVVTLWYRPPDVLFGAKLYSTSIDMWSAGCIFAELANAGRPLFPGNDVDDQLKRIFRLLGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPVQRISAEEALQHPYF
Aligned reference sequence
YEKL----------------EKIGEGT----------------YGTVFKAKN--RE------------------------------------THEIVALKRVR--LDDDDEGV--------------------------PSSALREICLLKEL--------------------------KHKNIVRLHDVLHSDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTLVFEFCD---------------QDLKKYFDS--CNG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLDPEIVKSFLFQLLKGLGFCHSR---------------------------------------------NVLHRDL-----KPQNLLINR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NGELKLADFGLARAFGIPVRC--Y---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SAEVVTLWYRPPDVLFG--A-----------------------------KLYS-TSIDMWSAGCIFAELANA--G--------------RPLFPG--------------------------------------------------NDVDDQLKRIFRL--LGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLN-------------------------------------------------------------------------ATGRDLLQNLLKCNPVQRIS--------------AEEALQHPYF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YEKL----------------EKI--------------------YGTVFKAKN--RE------------------------------------THEIVALKRVR--LD----GV--------------------------PSSALREICLLKEL--------------------------KHKNIVRLHDVLHSDK--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLTLVFEFCD---------------QDLKKYFDS--CNG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DLDPEIVKSFLFQLLKGLGFCHSR---------------------------------------------NVLHRDL-----KPQNLLINR--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NGELKLADFGLARAFGIPVRC--Y---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SAEVVTLWYRPPDVLFG--A-----------------------------KLYS-TSIDMWSAGCIFAELANA--A--------------RPLFPG--------------------------------------------------NDVDDQLKRIFRL--LGTPTEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLN-------------------------------------------------------------------------ATGRDLLQNLLKCNPVQRIS--------------AEEALQHPYF
Activation segment
DFGLARAFGIPVRC--Y---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------SAEVVTLWYRPPD
Binding pocket
EKI____YGTVFKVALKRVSALREICLLKELKKNIVRLHDVTLVFEFCD_QDLKKYFDSFCHSRNVLHRDLKPQNLLILADFGLA
Ligand info
Orthosteric ligand
None
Allosteric ligand
None
Ligand typeNo_ligand
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 1H4L, Chain B