1CM8 Chain A
Mitogen-activated protein kinase 12 (MAPK12)
Active — 53.9%DFG-inαC-inATPlike · ANP
Resolution
2.4 Å
R-value
0.232
Predicted activity confidence53.9%
Structure info
Alternate conformation—
Missing atoms12
Missing residues6
Salt bridge (KinCore)Saltbr-in
Sequence info
PDB sequence
RSGFYRQEVTKTAWEVRAVYRDLQPVAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDFYLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKGLRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLARQADSEMGVVTRWYRAPEVILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYFESLHQVQKYDDSRTLDEWKRVTYKEVLSFKP
UniProt reference sequence
YRDLQPVGSGAYGAVCSAVDGRTGAKVAIKKLYRPFQSELFAKRAYRELRLLKHMRHENVIGLLDVFTPDETLDDFTDFYLVMPFMGTDLGKLMKHEKLGEDRIQFLVYQMLKGLRYIHAAGIIHRDLKPGNLAVNEDCELKILDFGLARQADSEMTGYVVTRWYRAPEVILNWMRYTQTVDIWSVGCIMAEMITGKTLFKGSDHLDQLKEIMKVTGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNASPLAVNLLEKMLVLDAEQRVTAGEALAHPYF
Aligned reference sequence
YRDL----------------QPVGSGA----------------YGAVCSAVD--GR------------------------------------TGAKVAIKKLY--RPFQSELF--------------------------AKRAYRELRLLKHM--------------------------RHENVIGLLDVFTPDE--TLDDFT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DFYLVMPFMG---------------TDLGKLMKH--E--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLGEDRIQFLVYQMLKGLRYIHAA---------------------------------------------GIIHRDL-----KPGNLAVNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCELKILDFGLARQADSEM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGYVVTRWYRAPEVILN--W-----------------------------MRYT-QTVDIWSVGCIMAEMITG-----------------KTLFKG--------------------------------------------------SDHLDQLKEIMKV--TGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNAS-----------------------------------------------------------------------PLAVNLLEKMLVLDAEQRVT--------------AGEALAHPYF
Shaded segments mark residues present in the UniProt reference but not resolved in this structure.
Projected alignment
YRDL----------------QPV----------------------AVCSAVD--GR------------------------------------TGAKVAIKKLY--RPFQSELF--------------------------AKRAYRELRLLKHM--------------------------RHENVIGLLDVFTPDE--TLDDFT------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DFYLVMPFMG---------------TDLGKLMKH--E--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KLGEDRIQFLVYQMLKGLRYIHAA---------------------------------------------GIIHRDL-----KPGNLAVNE--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DCELKILDFGLARQADSEM----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GVVTRWYRAPEVILN--W-----------------------------MRYT-QTVDIWSVGCIMAEMITG-----------------KTLFKG--------------------------------------------------SDHLDQLKEIMKV--TGTPPAEFVQRLQSDEAKNYMKGLPELEKKDFASILTNAS-----------------------------------------------------------------------PLAVNLLEKMLVLDAEQRVT--------------AGEALAHPYF
Activation segment
DFGLARQADSEM----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GVVTRWYRAPE
Binding pocket
QPV______AVCSVAIKKLRAYRELRLLKHMRENVIGLLDVYLVMPFMG_TDLGKLMKHYIHAAGIIHRDLKPGNLAVILDFGLA
Ligand info
Orthosteric ligand
ANP
Allosteric ligand
None
Ligand typeATPlike
Consensus conformation
DFG conformationin
αC-helix conformationin
Predicted activityactive
KinaDB — structure detail · data from Browse_each_pdb_structure.csv · PDB 1CM8, Chain A